概述

  • 产品名称Anti-C1orf105抗体
    参阅全部 C1orf105 一抗
  • 描述
    兔多克隆抗体to C1orf105
  • 经测试应用适用于: ICC/IF, IHC-P, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    antigen sequence, corresponding to amino acids 93-176 (QPRTMKIPDD PKASFENCMS YRMSLHQPKF QTTPEPFHDD IPTESIHYRL PILGPRTAVF HGLLTEAYKT LKERQRSSLP RKEP) of Human C1orf105.

  • 阳性对照
    • Human kidney tissue; Transfected HEK293T cell lysate.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

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应用

Our Abpromise guarantee covers the use of ab121993 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/250 - 1/500.

靶标

Anti-C1orf105 antibody 图像

  • Immunofluorescent staining of Human cell line U-251 MG shows positivity in nucleoli. Recommended concentration of ab121993 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • All lanes : Anti-C1orf105 antibody (ab121993) at 1/250 dilution

    Lane 1 : Negative control (vector-only transfected HEK293T lysate)
    Lane 2 : C1orf105-transfected HEK293T overexpression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa))

    Developed using the ECL technique
  • ab121993, at 1/400, staining C1orf105 in paraffin-embedded Human kidney by Immunohistochemistry.

Anti-C1orf105 antibody (ab121993)参考文献

ab121993 has not yet been referenced specifically in any publications.

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