The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Detects a band of approximately 32 kDa (predicted molecular weight: 36 kDa).
功能Probable chaperone required for the generation of 1 O-glycan Gal-beta1-3GalNAc-alpha1-Ser/Thr (T antigen), which is a precursor for many extended O-glycans in glycoproteins. Probably acts as a specific molecular chaperone assisting the folding/stability of core 1 beta-3-galactosyltransferase (C1GALT1).
组织特异性Ubiquitously expressed. Abundantly expressed in salivary gland, stomach, small intestine, kidney, and testis and at intermediate levels in whole brain, cerebellum, spinal cord, thymus, spleen, trachea, lung, pancreas, ovary, and uterus.
疾病相关Defects in C1GALT1C1 are the cause of Tn syndrome (TNSYN) [MIM:300622]. Tn syndrome is a rare autoimmune disease caused by somatic mutation in the C1GALT1C1 gene in which subpopulations of blood cells of all lineages carry an incompletely glycosylated membrane glycoprotein, i.e. the Tn antigen. Since leukocytes and platelets are affected as well as red cells, anemia, leukopenia and thrombocytopenia are features. Tn-polyagglutinability is sometimes associated with leukemia or is a preleukemic state.
序列相似性Belongs to the glycosyltransferase 31 family. Beta3-Gal-T subfamily.
Immunocytochemistry/ Immunofluorescence - Anti-C1GALT1C1 antibody (ab68310)This image is courtesy of an Abreview submitted by Armen Petrosyan
ab68310 staining C1GALT1C1 in the Human Panc-1 cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and blocked with 1% serum for 60 minutes at 22°C. Samples were incubated with primary antibody (1/100 in PBST + 1% Donkey serum) for 1 hour at 22°C. An undiluted Alexa Fluor®488-conjugated Donkey anti-mouse polyclonal was used as the secondary antibody. Key Green-C1GALT1C1, Red-ab24586 Giantin-Golgi marker, Blue-Nucleus (DAPI)