• 产品名称Anti-C1GALT1C1抗体
    参阅全部 C1GALT1C1 一抗
  • 描述
    小鼠多克隆抗体to C1GALT1C1
  • 特异性This antibody is reactive against mammalian transfected lysate.
  • 经测试应用适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    C1GALT1C1 (NP_001011551.1, 1 a.a. ~ 318 a.a) full-length human protein.

  • 阳性对照
    • C1GALT1C1 transfected 293T cell lysate an human liver tissue lysate



Our Abpromise guarantee covers the use of ab68310 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 32 kDa (predicted molecular weight: 36 kDa).
ICC/IF 1/100.


  • 功能Probable chaperone required for the generation of 1 O-glycan Gal-beta1-3GalNAc-alpha1-Ser/Thr (T antigen), which is a precursor for many extended O-glycans in glycoproteins. Probably acts as a specific molecular chaperone assisting the folding/stability of core 1 beta-3-galactosyltransferase (C1GALT1).
  • 组织特异性Ubiquitously expressed. Abundantly expressed in salivary gland, stomach, small intestine, kidney, and testis and at intermediate levels in whole brain, cerebellum, spinal cord, thymus, spleen, trachea, lung, pancreas, ovary, and uterus.
  • 疾病相关Defects in C1GALT1C1 are the cause of Tn syndrome (TNSYN) [MIM:300622]. Tn syndrome is a rare autoimmune disease caused by somatic mutation in the C1GALT1C1 gene in which subpopulations of blood cells of all lineages carry an incompletely glycosylated membrane glycoprotein, i.e. the Tn antigen. Since leukocytes and platelets are affected as well as red cells, anemia, leukopenia and thrombocytopenia are features. Tn-polyagglutinability is sometimes associated with leukemia or is a preleukemic state.
  • 序列相似性Belongs to the glycosyltransferase 31 family. Beta3-Gal-T subfamily.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • HSPC067 antibody
    • 3-galactosyltransferase 2 antibody
    • Beta 1,3 galactosyltransferase 2 antibody
    • Beta1,3 galactosyltransferase 2 antibody
    • C1Gal T2 antibody
    • C1Gal-T2 antibody
    • C1GALT1 specific chaperone 1 antibody
    • C1GALT1-specific chaperone 1 antibody
    • C1galt1c1 antibody
    • C1GalT2 antibody
    • C1GLC_HUMAN antibody
    • C38H2 L1 antibody
    • C38H2 like protein 1 antibody
    • C38H2-L1 antibody
    • C38H2-like protein 1 antibody
    • C38H2L1 antibody
    • Core 1 beta1 antibody
    • Core 1 beta3 galactosyltransferase specific molecular chaperone antibody
    • Core 1 beta3-Gal-T2 antibody
    • Core 1 beta3-galactosyltransferase-specific molecular chaperone antibody
    • Core 1 UDP galactose:N acetylgalactosamine alpha R beta 1,3 galactosyltransferase 2 antibody
    • COSMC antibody
    • MGC19947 antibody
    • MST143 antibody
    see all

Anti-C1GALT1C1 antibody 图像

  • Anti-C1GALT1C1 antibody (ab68310) at 1/500 dilution + human liver tissue lysate at 25 µg

    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 36 kDa
    Observed band size : 32 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-C1GALT1C1 antibody (ab68310) at 1/500 dilution

    Lane 1 : C1GALT1C1 transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 36 kDa
    Observed band size : 32 kDa (why is the actual band size different from the predicted?)
  • ab68310 staining C1GALT1C1 in the Human Panc-1 cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and blocked with 1% serum for 60 minutes at 22°C. Samples were incubated with primary antibody (1/100 in PBST + 1% Donkey serum) for 1 hour at 22°C. An undiluted Alexa Fluor®488-conjugated Donkey anti-mouse polyclonal was used as the secondary antibody.
    Key Green-C1GALT1C1, Red-ab24586 Giantin-Golgi marker, Blue-Nucleus (DAPI)

    See Abreview

Anti-C1GALT1C1 antibody (ab68310)参考文献

ab68310 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Panc-1)
Specification Panc-1
Fixative Paraformaldehyde
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C

Dr. Armen Petrosyan

Verified customer

提交于 Mar 11 2013