概述

  • 产品名称
    Anti-C1GALT1抗体
    参阅全部 C1GALT1 一抗
  • 描述
    小鼠单克隆抗体to C1GALT1
  • 经测试应用
    适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant fragment: ETFHPFVPEH HLIKGYLPRT FWYWNYNYYP PVEGPGCCSD LAVSFHYVDS TTMYELEYLV YHLRPYGYLY RYQPTLPERI LKEISQANKN EDTKVKLGNP , corresponding to amino acids 264-364 of Human C1GALT1

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液
    Preservative: None
    PBS, pH 7.2
  • Concentration information loading...
  • 纯度
    Protein G purified
  • 克隆
    单克隆
  • 同种型
    IgG2a
  • 轻链类型
    kappa
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab57492 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 42 kDa.
ICC/IF Use at an assay dependent concentration.

靶标

  • 相关性
    C1GALT1 (Core 1 synthase, glycoprotein-N-acetylgalactosamine 3-beta-galactosyltransferase, 1) is a glycosyltransferase that generates the core 1 O-glycan Gal-beta1-3GalNAc-alpha1-Ser/Thr (T antigen), which is synthesized by the transfer of Gal from UDP-Gal to GalNAc-alpha-1-R. Core 1 is a precursor for many extended O-glycans in glycoproteins. C1GALT1 plays a central role in many processes, such as angiogenesis, thrombopoiesis and kidney homeostasis development.
  • 细胞定位
    Membrane; Single-pass type II membrane protein.
  • 数据库链接
  • 别名
    • B3Gal T8 antibody
    • Beta 1 3 galactosyltransferase antibody
    • C1GALT antibody
    • Core 1 beta1 3 galactosyltransferase 1 antibody
    • Core 1 beta3 Gal T antibody
    • Core 1 O glycan T synthase antibody
    • Core 1 synthase glycoprotein N acetylgalactosamine 3 beta galactosyltransferase 1 antibody
    • Core 1 UDP galactose:N acetylgalactosamine alpha R beta 1 3 galactosyltransferase antibody
    • Glycoprotein N acetylgalactosamine 3 beta galactosyltransferase 1 antibody
    • T synthase antibody
    see all

Anti-C1GALT1 antibody 图像



  • Predicted band size : 42 kDa
  • Immunofluorescence analysis of Panc1-bC2GnT-M cells, staining C1GALT1 with ab57492.

    Cells were fixed in paraformaldehyde before incubating with primary antibody (1/100) for 1 hour. A DyLight®488-conjugated donkey anti-mouse IgG (1/200) was used as the secondary antibody.

Anti-C1GALT1 antibody (ab57492)参考文献

This product has been referenced in:
  • Petrosyan A  et al. Keratin 1 plays a critical role in golgi localization of core 2 N-acetylglucosaminyltransferase M via interaction with its cytoplasmic tail. J Biol Chem 290:6256-69 (2015). Read more (PubMed: 25605727) »
  • Petrosyan A  et al. Restoration of compact Golgi morphology in advanced prostate cancer enhances susceptibility to galectin-1-induced apoptosis by modifying mucin O-glycan synthesis. Mol Cancer Res 12:1704-16 (2014). WB ; Human . Read more (PubMed: 25086069) »

See all 4 Publications for this product

Product Wall

Application
Western blot
Loading amount
25 µg
Gel Running Conditions
Reduced Denaturing (8% SDS-PAGE)
Sample
Human Cell lysate - whole cell (LNCaP)
Specification
LNCaP
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Dr. Armen Petrosyan

Verified customer

提交于 Nov 21 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (A549)
Specification
A549
Fixative
Formaldehyde
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Username

Dr. Armen Petrosyan

Verified customer

提交于 Apr 28 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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