概述

  • 产品名称
    Anti-C1GALT1抗体
    参阅全部 C1GALT1 一抗
  • 描述
    小鼠单克隆抗体to C1GALT1
  • 经测试应用
    适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant fragment: ETFHPFVPEH HLIKGYLPRT FWYWNYNYYP PVEGPGCCSD LAVSFHYVDS TTMYELEYLV YHLRPYGYLY RYQPTLPERI LKEISQANKN EDTKVKLGNP , corresponding to amino acids 264-364 of Human C1GALT1

  • 常规说明

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by July 2018 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. 

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液
    Preservative: None
    PBS, pH 7.2
  • Concentration information loading...
  • 纯度
    Protein G purified
  • 克隆
    单克隆
  • 同种型
    IgG2a
  • 轻链类型
    kappa
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab57492 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 42 kDa.
ICC/IF Use at an assay dependent concentration.

靶标

  • 相关性
    C1GALT1 (Core 1 synthase, glycoprotein-N-acetylgalactosamine 3-beta-galactosyltransferase, 1) is a glycosyltransferase that generates the core 1 O-glycan Gal-beta1-3GalNAc-alpha1-Ser/Thr (T antigen), which is synthesized by the transfer of Gal from UDP-Gal to GalNAc-alpha-1-R. Core 1 is a precursor for many extended O-glycans in glycoproteins. C1GALT1 plays a central role in many processes, such as angiogenesis, thrombopoiesis and kidney homeostasis development.
  • 细胞定位
    Membrane; Single-pass type II membrane protein.
  • 数据库链接
  • 别名
    • B3Gal T8 antibody
    • Beta 1 3 galactosyltransferase antibody
    • C1GALT antibody
    • Core 1 beta1 3 galactosyltransferase 1 antibody
    • Core 1 beta3 Gal T antibody
    • Core 1 O glycan T synthase antibody
    • Core 1 synthase glycoprotein N acetylgalactosamine 3 beta galactosyltransferase 1 antibody
    • Core 1 UDP galactose:N acetylgalactosamine alpha R beta 1 3 galactosyltransferase antibody
    • Glycoprotein N acetylgalactosamine 3 beta galactosyltransferase 1 antibody
    • T synthase antibody
    see all

图片



  • Predicted band size : 42 kDa
  • Immunofluorescence analysis of Panc1-bC2GnT-M cells, staining C1GALT1 with ab57492.

    Cells were fixed in paraformaldehyde before incubating with primary antibody (1/100) for 1 hour. A DyLight®488-conjugated donkey anti-mouse IgG (1/200) was used as the secondary antibody.

文献

This product has been referenced in:
  • Ho CW  et al. The cytokine-cosmc signaling axis upregulates the tumor-associated carbohydrate antigen Tn. Oncotarget 7:61930-61944 (2016). WB, IF . Read more (PubMed: 27542280) »
  • Petrosyan A  et al. Keratin 1 plays a critical role in golgi localization of core 2 N-acetylglucosaminyltransferase M via interaction with its cytoplasmic tail. J Biol Chem 290:6256-69 (2015). Read more (PubMed: 25605727) »

See all 5 Publications for this product

客户评价及客户问答

Application
Western blot
Loading amount
25 µg
Gel Running Conditions
Reduced Denaturing (8% SDS-PAGE)
Sample
Human Cell lysate - whole cell (LNCaP)
Specification
LNCaP
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Dr. Armen Petrosyan

Verified customer

提交于 Nov 21 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (A549)
Specification
A549
Fixative
Formaldehyde
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Username

Dr. Armen Petrosyan

Verified customer

提交于 Apr 28 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册