概述

  • 产品名称Anti-C19orf61抗体
  • 描述
    兔多克隆抗体to C19orf61
  • 经测试应用适用于: IP, IHC-P, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant
  • 免疫原

    Synthetic peptide designed within residues corresponding to amino acids 75 - 125 of Human C19orf61 (NP_061981.2).

  • 阳性对照
    • HeLa cell lysate

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.09% Sodium Azide
    Constituents: Tris citrate/phosphate buffer, pH 7-8
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

相关产品

应用

Our Abpromise guarantee covers the use of ab85659 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IP Use at 5-10 µg/mg of lysate.
IHC-P 1/2000 - 1/10000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 58 kDa.

Not suitable for crude preparations (e.g. whole cell lysate). Can be used for WB of enriched (e.g. immunoprecipitated) sources of C19orf61.

靶标

  • 功能Involved in nonsense-mediated decay (NMD) of mRNAs containing premature stop codons. Is recruited by release factors to stalled ribosomes together with SMG1 and SMG8 (forming the SMG1C protein kinase complex) and, in the SMG1C complex, is required for the efficient association between SMG1 and SMG8.
  • 序列相似性Belongs to the SMG9 family.
  • 翻译后修饰Phosphorylated by SMG1.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Chromosome 19 open reading frame 61 antibody
    • DKFZp564H1322 antibody
    • F17127_1 antibody
    • FLJ12886 antibody
    • Protein smg 9 homolog antibody
    • Protein smg-9 homolog antibody
    • Protein SMG9 antibody
    • SMG 9 antibody
    • smg9 antibody
    • SMG9_HUMAN antibody
    • Suppressor with morphogenetic effect on genitalia protein 9, antibody
    see all

Anti-C19orf61 antibody 图像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling C19orf61 with ab85689 at 1/5000 (0.2µg/ml). Detection: DAB.
  • 1mg whole cell lysate from HeLa cells was immunoprecipitated using 10µg ab90659.
    20% of the immunoprecipitate was loaded in each lane, and probed with ab90659 at 1µg/ml (lane 1) or with a control IgG (lane 2).
    Detection: chemoluminescence, with an exposure time of 1 second.

Anti-C19orf61 antibody (ab85659)参考文献

ab85659 has not yet been referenced specifically in any publications.

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