Rabbit, Horse, Cow, Pig, Chimpanzee, Rhesus monkey, Gorilla, Opossum, Orangutan
Synthetic peptide corresponding a region between residues 250 and 300 of human C17orf71 (NP_060619.4)
Whole cell lysate from HeLa cells.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium Azide
Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8
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Immunogen affinity purified
Antibody was affinity purified using an epitope specific to C17orf71 immobilized on solid support.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Use at 2-5 µg/mg of lysate.
Is unsuitable for WB.
C17orf71 belongs to the UPF0487 family. There are 3 isoforms produced by alternative splicing.
ABC 2 antibody
Amplified in breast cancer gene 2 protein antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C17orf71 antibody (ab80472)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling C17orf71 with ab80472 at 1/1000 (1µg/ml). Detection: DAB.
Immunoprecipitation - Anti-C17orf71 antibody (ab80472)
Detection of human C17orf71 by Western Blot of
Immunoprecipitates in whole cell lysates (1 mg for IP, 20% of IP loaded) from HeLa cells (lane 1), using 3 µg/mg of lysate of ab80472 for IP.
Lane 2: Control IgG.
For blotting immunoprecipitated C17orf71, ab80472 was used at 1 µg/ml.
Detection: via Chemiluminescence with an exposure time of 10 seconds.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"