Synthetic peptide, corresponding to a region within N terminal amino acids 72-121 (CGEEKSSDFS GEKKVGRKSL QVQQHSKRTE IIPPFLKLSK EKVTRKENSL) of human C17orf57 (NP_689560).
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Constituents: 2% Sucrose, PBS
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Immunogen affinity purified
Purified by peptide affinity chromatography method.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1 µg/ml. Predicted molecular weight: 110 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
C17orf57 contains six EF-hand domains. There are two named isoforms. The function of this protein is not known.
- Chromosome 17 open reading frame 57 antibody
- FLJ40342 antibody
- Hypothetical protein LOC124989 antibody
Western blot - C17orf57 antibody (ab85494)
Anti-C17orf57 antibody (ab85494) at 1 µg/ml (in 5% skim milk / PBS buffer) + 293T cell lysate at 10 µg
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size: 110 kDa
Observed band size: 110 kDa
Additional bands at: 144 kDa, 65 kDa (possible isoform). We are unsure as to the identity of these extra bands.
has not yet been referenced specifically in any publications.
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