by a Cysteine residue linker, corresponding to C terminal amino acids 3268-3278 of Human C17orf27
This antibody is raised against a protein that has limited or no commercial antibodies available thus far. There are no data on the expression of the protein in the range of common cell lines and tissues available to us.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
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Immunogen affinity purified
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 2.5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Note=A chromosomal aberration involving ALO17 is associated with anaplastic large-cell lymphoma (ALCL). Translocation t(2;17)(p23;q25) with ALK.
Information by UniProt
ALK lymphoma oligomerization partner on chromosome 17 antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - C17orf27 antibody (ab58580)
ab58580 (2.5 µg/ml) staining C17orf27 in human kidney by IHC-P. Steamed antigen retrieval in citrate buffer pH 6, AP-staining. The image shows rare staining of nucleoli across the kidney .
has not yet been referenced specifically in any publications.
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