Anti-Treslin抗体(ab124268)
Key features and details
- Rabbit polyclonal to Treslin
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-Treslin抗体
参阅全部 Treslin 一抗 -
描述
兔多克隆抗体to Treslin -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human
预测可用于: Horse, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan -
免疫原
Synthetic peptide corresponding to Human Treslin aa 950-1050.
Database link: Q7Z2Z1 -
阳性对照
- WB: 293T whole cell lysate
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
ab124268 was affinity purified using an epitope specific to Treslin immobilized on solid support. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab124268于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB | (1) |
1/500 - 1/2500. Predicted molecular weight: 211 kDa.
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说明 |
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WB
1/500 - 1/2500. Predicted molecular weight: 211 kDa. |
靶标
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功能
Regulator of DNA replication and S/M and G2/M checkpoints. Regulates the triggering of DNA replication initiation via its interaction with TOPBP1 by participating to CDK2-mediated loading of CDC45L onto replication origins. Required for the transition from pre-replication complex (pre-RC) to pre-initiation complex (pre-IC). Required to prevent mitotic entry after treatment with ionizing radiation. -
序列相似性
Belongs to the treslin family. -
细胞定位
Nucleus. Associates with chromatin. - Information by UniProt
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数据库链接
- Entrez Gene: 90381 Human
- Omim: 613298 Human
- SwissProt: Q7Z2Z1 Human
- Unigene: 441708 Human
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别名
- C15orf42 antibody
- chromosome 15 open reading frame 42 antibody
- replication-stimulating protein antibody
see all
图片
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All lanes : Anti-Treslin antibody (ab124268) at 1/1000 dilution
Lane 1 : 293 whole cell lysate treated with control siLUC RNAi
Lanes 2-3 : 293 whole cell lysate treated with RNAi targeting Treslin
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : Donkey Anti-Rabbit IgG H&L (HRP) (ab16284) at 1/20000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 211 kDa
Observed band size: 250 kDa why is the actual band size different from the predicted?
Additional bands at: 130 kDa (possible non-specific binding)
Exposure time: 2 minutesBlocking: 5% milk
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All lanes : Anti-Treslin antibody (ab124268) at 0.4 µg/ml
Lane 1 : 293T whole cell lysate at 50 µg
Lane 2 : 293T whole cell lysate at 15 µg
Developed using the ECL technique.
Predicted band size: 211 kDa
Exposure time: 3 minutes
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab124268 尚未被引用在任何文献中。