概述

  • 产品名称Anti-C14orf68抗体
  • 描述
    山羊多克隆抗体to C14orf68
  • 经测试应用适用于: WB, ELISA, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Dog
  • 免疫原

    Synthetic peptide:

    C-KSRLQADGQGQRR

    , corresponding to internal sequence amino acids 242-254 of Human C14orf68 (NP_997000.2).

  • 阳性对照
    • Human Liver tissue.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明ab77196 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

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应用

Our Abpromise guarantee covers the use of ab77196 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
ELISA
IHC-P
  • 应用说明Peptide ELISA: Use at an assay dependent dilution. Antibody detection limit dilution 1/32,000.
    IHC-P: Use at a concentration of 2.5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
    WB: Use at a concentration of 0.5 µg/ml. Predicted molecular weight: 33 kDa.
    Western Blot: Preliminary experiments gave an approx 22kDa band in Human Liver lysates after 0.5µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 33.4kDa according to NP_997000.2. The 22kDa band was successfully blocked by incubation with the immunizing peptide.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    Anti-C14orf68 antibody 图像

    • ab77196 at 2.5µg/ml staining C14orf68 in human liver by Immunohistochemistry, paraffin-embedded tissue. Note staining of nucleoli in select hepatocytes.

    Anti-C14orf68 antibody (ab77196)参考文献

    ab77196 has not yet been referenced specifically in any publications.

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