Anti-C14ORF21抗体(ab86119)

概述

  • 产品名称Anti-C14ORF21抗体
    参阅全部 C14ORF21 一抗
  • 描述
    兔多克隆抗体to C14ORF21
  • 经测试应用适用于: ICC/IF, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Rabbit, Cow
  • 免疫原

    Synthetic peptide, corresponding to a region within internal sequence amino acids 215-264 (GTILESERAR PRGSQSSEAQ KTPAQECKPA DFEVPETFLN RLQDLSSSFL) of Human C14ORF21, (NP_777573)

  • 阳性对照
    • Hela cell lysate This antibody gave a positive result when used in the following methanol fixed cell lines: HepG2.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab86119 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 69 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 相关性The specific function of C14orf21 is not yet known. It contains 6 pumilio repeats.
  • 数据库链接
  • 别名
    • chromosome 14 open reading frame 21 antibody
    • hypothetical protein LOC161424 antibody
    • KIAA2021 antibody
    • NOP9 antibody
    • NOP9 nucleolar protein antibody
    • NOP9 nucleolar protein homolog antibody
    • nucleolar protein 9 antibody
    • Pumilio domain containing protein C14orf21 antibody
    see all

Anti-C14ORF21 antibody 图像

  • Anti-C14ORF21 antibody (ab86119) at 1 µg/ml + Hela cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 69 kDa
    Observed band size : 80 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 90 kDa. We are unsure as to the identity of these extra bands.
  • ICC/IF image of ab86119 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab86119 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-C14ORF21 antibody (ab86119)参考文献

ab86119 has not yet been referenced specifically in any publications.

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Thank you for providing that extra information in relation to anti-C14ORF21 antibody (ab86119).

Unfortunately, we have not tried using this antibody in staining of cells, it has only been validated in Western blotting as yet. This does not me...

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