Anti-C14orf106抗体(ab89265)
Key features and details
- Mouse polyclonal to C14orf106
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-C14orf106抗体 -
描述
小鼠多克隆抗体to C14orf106 -
宿主
Mouse -
经测试应用
适用于: WB, ICC/IFmore details -
种属反应性
与反应: Human -
免疫原
Recombinant full length protein within Human C14orf106. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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阳性对照
- WB: Human spleen tissue lysate, C14orf106 transfected 293T cell lysate ICC/IF: HeLa cell
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.40
Constituent: 100% PBS -
Concentration information loading...
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纯度
Protein A purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab89265于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB | ||
ICC/IF |
说明 |
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WB: Use at a concentration of 1 µg/ml. Predicted molecular weight: 129 kDa for isoform 1 and 44 kDa for isoform 2.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
靶标
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相关性
C14orf106 is required for recruitment of CENPA to centromeres and normal chromosome segregation during mitosis. It interacts with SP1. There are two isoforms. -
细胞定位
Nucleus. Centromere. Note: Associated with centromeres in interphase cells, from late anaphase to the G1 phase. Not detected on centromeres during earlier phases of mitosis. Associated with chromatin. -
数据库链接
- Entrez Gene: 55320 Human
- SwissProt: Q6P0N0 Human
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别名
- Chromosome 14 open reading frame 106 antibody
- HsKNL-2 antibody
- Kinetochore-associated protein KNL-2 homolog antibody
see all
图片
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Anti-C14orf106 antibody (ab89265) at 1 µg/ml + human spleen tissue lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 129 , 44 kDa
Observed band size: ~129 kDa why is the actual band size different from the predicted? -
All lanes : Anti-C14orf106 antibody (ab89265) at 1 µg/ml
Lane 1 : C14orf106 transfected 293T cell lysate
Lane 2 : Non transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Developed using the ECL technique.
Predicted band size: 129 , 44 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted? -
ab89265, at 10 µg/ml, staining C14orf106 in HeLa cell by Immunofluorescence.
实验方案
数据表及文件
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Datasheet download
文献 (0)
ab89265 尚未被引用在任何文献中。