概述

  • 产品名称Anti-C13orf8抗体
    参阅全部 C13orf8 一抗
  • 描述
    兔多克隆抗体to C13orf8
  • 特异性This antibody recognizes C13orf8
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    A region within synthetic peptide: PAASPESRKS ARTTSPEPRK PSPSESPEPW KPFPAVSPEP RRPAPAVSPG, corresponding to internal sequence amino acids 261-310 of Human C13orf8

  • 阳性对照
    • Daudi cell lysate. Human skeletal muscle cells.

性能

应用

Our Abpromise guarantee covers the use of ab48703 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 89 kDa (predicted molecular weight: 89 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use at an assay dependent concentration.

靶标

  • 功能Required for proper alignment of chromosomes at metaphase and their accurate segregation during mitosis. Involved in the maintenance of spindle microtubules attachment to the kinetochore during sister chromatid biorientation. May recruit CENPE and CENPF to the kinetochore.
  • 序列相似性Contains 1 C2H2-type zinc finger.
  • 翻译后修饰Phosphorylated by CDK1. Mitotic phosphorylation is required for the attachment of spindle microtubules to the kinetochore.
  • 细胞定位Nucleus. Chromosome. Chromosome > centromere > kinetochore. Cytoplasm > cytoskeleton > spindle.
  • Information by UniProt
  • 数据库链接
  • 别名
    • C13orf8 antibody
    • CAMP antibody
    • CHAMP antibody
    • CHAMP1 antibody
    • CHAP1_HUMAN antibody
    • chromosome 13 open reading frame 8 antibody
    • Chromosome alignment-maintaining phosphoprotein 1 antibody
    • FLJ90413 antibody
    • KIAA1802 antibody
    • Zinc finger protein 828 antibody
    • Zinc finger protein KIAA1802 antibody
    • ZNF828 antibody
    see all

Anti-C13orf8 antibody 图像

  • Anti-C13orf8 antibody (ab48703) at 0.25 µg/ml + Daudi cell lysate at 10 µg with 5% skimmed milk in PBS

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 89 kDa
    Observed band size : 89 kDa
  • ab48703 (4-8µg/ml) staining of Human muscle tissue cells (10µg) with positively stained skeletal muscle cells (arrows). Magnification: x400

Anti-C13orf8 antibody (ab48703)参考文献

ab48703 has not yet been referenced specifically in any publications.

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