The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000. Detects a band of approximately 90 kDa (predicted molecular weight: 90 kDa).
Use at 2-5 µg/mg of lysate.
Mediator of progesterone that by acting on the phospholipase A2 enzyme interferes with arachidonic acid metabolism, induces a Th2 biased immune response, and by controlling NK activity exerts an anti-abortive effect.
Expressed at highest levels in testis. Moderate expression is detected in spleen, thymus, prostate, ovary, small intestine, and colon.
All lanes : Anti-C13orf24 antibody (ab72118) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 5 µg Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 90 kDa Observed band size: 90 kDa
Exposure time: 30 seconds
Immunoprecipitation - C13orf24 antibody (ab72118)
Detection of C13orf24 by Western Blot of Immunprecipitate.
ab72118 at 1µg/ml staining C13orf24 in HeLa whole cell lysate immunoprecipitated using ab72118 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 10 seconds.