概述

  • 产品名称
    Anti-C12orf48抗体- C-terminal
    参阅全部 C12orf48 一抗
  • 描述
    兔多克隆抗体to C12orf48 - C-terminal
  • 经测试应用
    适用于: ICC/IF, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide conjugated to KLH, between amino acids 529-558 from the C terminal region of Human C12orf48 (NP_060385.3).

  • 阳性对照
    • A549 cell line lysate. This antibody gave a positive result when used in the following methanol fixed cell line: HeLa.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
  • 存储溶液
    Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 纯化说明
    ab107308 is purified through a protein A column, followed by peptide affinity purification.
  • 克隆
    多克隆
  • 同种型
    IgG

应用

Our Abpromise guarantee covers the use of ab107308 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml.
WB 1/100 - 1/500. Predicted molecular weight: 65 kDa.

靶标

  • 功能
    May be involved in RNA metabolism. Binds single-strand DNA and poly(A) homopolymers (By similarity). Positively regulate the poly(ADP-ribosyl)ation activity of PARP1.
  • 组织特异性
    Restricted to testis. Overexpressed in multiple cancer cells.
  • 序列相似性
    Belongs to the PARP1BP family.
  • 细胞定位
    Cytoplasm. Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AROM antibody
    • C12orf48 antibody
    • Chromosome 12 open reading frame 48 antibody
    • FLJ20641 antibody
    • PARP 1 binding protein antibody
    • PARP-1 binding protein antibody
    • PARP1 binding protein antibody
    • PARP1-binding protein antibody
    • PARPBP antibody
    • PR1BP_HUMAN antibody
    see all

Anti-C12orf48 antibody - C-terminal 图像

  • ICC/IF image of ab107308 stained HeLa cells.  The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab107308 at 5µg/mL overnight at +4°C. The secondary antibody (green) was DyLight® 488 Goat anti-Rabbit IgG (H+L) (ab96899) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Anti-C12orf48 antibody - C-terminal (ab107308) at 1/100 dilution + A549 cell line lysate at 35 µg

    Predicted band size : 65 kDa

Anti-C12orf48 antibody - C-terminal (ab107308)参考文献

This product has been referenced in:
  • Hampp S  et al. DNA damage tolerance pathway involving DNA polymerase ? and the tumor suppressor p53 regulates DNA replication fork progression. Proc Natl Acad Sci U S A 113:E4311-9 (2016). WB ; Human . Read more (PubMed: 27407148) »

See 1 Publication for this product

Product Wall

There are currently no Abreviews or Questions for ab107308.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册