Anti-C12orf48抗体- C-terminal (ab107308)

概述

  • 产品名称Anti-C12orf48抗体- C-terminal
    参阅全部 C12orf48 一抗
  • 描述
    兔多克隆抗体to C12orf48 - C-terminal
  • 经测试应用适用于: ICC/IF, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide conjugated to KLH, between amino acids 529-558 from the C terminal region of Human C12orf48 (NP_060385.3).

  • 阳性对照
    • A549 cell line lysate. This antibody gave a positive result when used in the following methanol fixed cell line: HeLa.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
  • 存储溶液Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明ab107308 is purified through a protein A column, followed by peptide affinity purification.
  • 克隆多克隆
  • 同种型IgG

应用

Our Abpromise guarantee covers the use of ab107308 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml.
WB 1/100 - 1/500. Predicted molecular weight: 65 kDa.

靶标

  • 功能May be involved in RNA metabolism. Binds single-strand DNA and poly(A) homopolymers (By similarity). Positively regulate the poly(ADP-ribosyl)ation activity of PARP1.
  • 组织特异性Restricted to testis. Overexpressed in multiple cancer cells.
  • 序列相似性Belongs to the PARP1BP family.
  • 细胞定位Cytoplasm. Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AROM antibody
    • C12orf48 antibody
    • Chromosome 12 open reading frame 48 antibody
    • FLJ20641 antibody
    • PARP 1 binding protein antibody
    • PARP-1 binding protein antibody
    • PARP1 binding protein antibody
    • PARP1-binding protein antibody
    • PARPBP antibody
    • PR1BP_HUMAN antibody
    see all

Anti-C12orf48 antibody - C-terminal 图像

  • ICC/IF image of ab107308 stained HeLa cells.  The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab107308 at 5µg/mL overnight at +4°C. The secondary antibody (green) was DyLight® 488 Goat anti-Rabbit IgG (H+L) (ab96899) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Anti-C12orf48 antibody - C-terminal (ab107308) at 1/100 dilution + A549 cell line lysate at 35 µg

    Predicted band size : 65 kDa

Anti-C12orf48 antibody - C-terminal (ab107308)参考文献

ab107308 has not yet been referenced specifically in any publications.

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