antigen, corresponding to amino acids 1456-1593 of Human C12orf35.
Human nasopharynx tissue.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.02% Sodium azide Constituents: 59% PBS, 40% Glycerol
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Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Information by UniProt
Uncharacterized protein C12orf35 antibody
Immunocytochemistry/ Immunofluorescence - Anti-C12orf35 antibody (ab121480)
Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleoli and cytoskeleton (intermediate filaments). Recommended concentration of ab121480 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C12orf35 antibody (ab121480)
ab121480, at 1/135 dilution, staining C12orf35 in paraffin-embedded Human nasopharinx tissue, by Immunohistochemistry.
has not yet been referenced specifically in any publications.
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