概述

  • 产品名称Anti-C12orf34抗体
    参阅全部 C12orf34 一抗
  • 描述
    兔多克隆抗体to C12orf34
  • 经测试应用适用于: WB, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    antigen sequence: PSIHSLLYQL NQQCQAPGAA PPACQGMAIP HPSPAKHGPV PSFPSMAYSA AAGLPDCRKG TELGQGATQA LTLAGAAKPA GYADSGLDYL LWPQKP, corresponding to amino acids 197-292 of Human C12orf34.

  • 阳性对照
    • Human stomach tssue; U 251 MG lysate.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

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应用

Our Abpromise guarantee covers the use of ab122626 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/250 - 1/500.
IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

靶标

Anti-C12orf34 antibody 图像

  • Immunofluorescent staining of Human cell line U-251 MG shows positivity in nucleus but not nucleoli, plasma membrane, mitochondria and focal adhesions. Recommended concentration of ab122626 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab122626 at 1/200 dilution staining C12orf34 in paraffin-embedded Human stomach tissue by Immunohistochemistry.
  • All lanes : Anti-C12orf34 antibody (ab122626) at 1/250 dilution

    Lane 1 : RT 4 lysate
    Lane 2 : U 251 MG lysate
    Lane 3 : Human plasma lysate
    Lane 4 : Human liver lysate
    Lane 5 : Human tonsil lysate

    Developed using the ECL technique

Anti-C12orf34 antibody (ab122626)参考文献

ab122626 has not yet been referenced specifically in any publications.

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