概述

  • 产品名称Anti-C12orf29抗体
    参阅全部 C12orf29 一抗
  • 描述
    兔多克隆抗体to C12orf29
  • 经测试应用适用于: IHC-P, ICC/IF, Flow Cyt, WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Sheep, Human
  • 免疫原

    Synthetic peptide conjugated to KLH, between amino acids 68-96 from the N-terminal region of Human C12orf29 (NP_001009894.2).

  • 阳性对照
    • NCI-H460 cell lysate, Mouse bladder tissue lysate

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
  • 存储溶液Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明ab107423 is purified through a protein A column, followed by peptide affinity purification.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

相关产品

应用

Our Abpromise guarantee covers the use of ab107423 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
WB 1/100 - 1/500. Predicted molecular weight: 37 kDa.Can be blocked with C12orf29 peptide (ab139423).

靶标

Anti-C12orf29 antibody 图像

  • Anti-C12orf29 antibody (ab107423) at 1/100 dilution + NCI-H460 cell line lysate at 35 µg

    Predicted band size : 37 kDa
  • Anti-C12orf29 antibody (ab107423) at 1/100 dilution + Mouse bladder tissue lysate at 35 µg

    Predicted band size : 37 kDa
  • Dectection of C12orf29 using ab107423 by immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
  • Confocal immunofluorescent analysis of C12orf29 using ab107423 in cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
  • C12orf29 antibody (ab107423) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Anti-C12orf29 antibody (ab107423)参考文献

This product has been referenced in:
  • Friis TE  et al. A Polymerase Chain Reaction-Based Method for Isolating Clones from a Complimentary DNA Library in Sheep. Tissue Eng Part C Methods N/A:N/A (2014). ICC/IF, IHC-P, WB ; Sheep, Human, Rat . Read more (PubMed: 24447069) »

See 1 Publication for this product

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Thank you for contacting us and your patience.

I was in contact with the lab who produces the antibody for us and this is their answer: Each lot is qualified with specific applications the antibody supports. In this case, for WB, IHC, IF and...

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Thank you for contacting us.

The blocking peptide datasheet has been added with catalogue number ab139423.

Also more QC datahas been added.

I hope this information is helpful to you. Please do not hesitate to contact us if yo...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"