antigen sequence: SYSQDMTGVTPFQMIFEVDEKPRTLMTDCLVIKHFLRKIIMVHPKVRFHF SVKVNGILSTEIFGVENEPTLNLGNGIALLVDSQHYVSRPNFGTIESHC, corresponding to amino acids 40-137 of Human C11orf80.
Human pancreas tissue; Human plasma, Human Liver lysate.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
1/10 - 1/20. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Perform Heat Induced Epitope Retrieval (HIER) at pH 6 using a pressure boiler as heat source.
1/250 - 1/500.
Information by UniProt
Chromosome 11 open reading frame 80 antibody
Immunocytochemistry/ Immunofluorescence - Anti-C11orf80 antibody (ab122519)
Immunofluorescent staining of Human cell line A-431 shows positivity in centrosome. Recommended concentration of ab122519 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C11orf80 antibody (ab122519)
ab122519 at 1/15 dilution staining C11orf80 in Paraffin-embedded Human Pancreas tissue by Immunohistochemistry.
Western blot - Anti-C11orf80 antibody (ab122519)
All lanes : Anti-C11orf80 antibody (ab122519) at 1/250 dilution Lane 1 : RT4 lysate Lane 2 : U251 MG lysate Lane 3 : Human plasma Lane 4 : Human liver lysate Lane 5 : Human tonsil lysate Developed using the ECL technique
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"