概述

  • 产品名称
    Anti-C11orf1抗体
    参阅全部 C11orf1 一抗
  • 描述
    兔多克隆抗体to C11orf1
  • 经测试应用
    适用于: IHC-P, WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    antigen sequence: QERYDLRNIV QPKPLPSQFG HYFETTYDTS YNNKMPLSTH RFKREPHWFP GHQPELDPPR YKCTEKSTYM NSYSKP, corresponding to C terminal amino acids 75-150 of Human C11orf1.

  • 阳性对照
    • Human hippocampus tissue; C11orf1 over-expression HEK293T lysate; U 251 MG cells.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab122472 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/250 - 1/500.
ICC/IF Use a concentration of 1 - 4 µg/ml. Fixation/Permeabilization: PFA/Triton X-100

靶标

图片

  • ab122472, at 1/200, staining C11orf1 in paraffin-embedded Human hippocampus tissue by Immunohistochemistry.
  • All lanes : Anti-C11orf1 antibody (ab122472) at 1/250 dilution

    Lane 1 : Negative control (vector only transfected HEK293T lysate)
    Lane 2 : Over-expression lysate (co-expressed with a C-terminal myc-DDK tag [~3.1 kDa] in HEK293T cells)

    Developed using the ECL technique
  • ab122472, at 4 µg/ml, staining C11orf1 in in PFA/Triton X-100 fixed/permeabilized U 251 MG cells by Immunofluorescence (green).

文献

ab122472 has not yet been referenced specifically in any publications.

客户评价及客户问答

There are currently no Customer reviews or Questions for ab122472.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册