概述

  • 产品名称Anti-C10orf53抗体
  • 描述
    兔多克隆抗体to C10orf53
  • 经测试应用适用于: WB, ICC/IF, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    antigen sequence: MPKNAVVILR YGPYSAAGLP VEHHTFRLQG LQAVLAIDGH EVILEKIEDW NVVELMVNEE VIFHCNIKDL EF, corresponding to N terminal amino acids 1-72 of Human C10orf53

  • 阳性对照
    • Human testis tissue

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

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应用

Our Abpromise guarantee covers the use of ab122426 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/100 - 1/250.
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

Anti-C10orf53 antibody 图像

  • Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus but not nucleoli and cytoplasm. Recommended concentration of ab122426 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • Lane 1 :
    Lanes 2 - 3 : Anti-C10orf53 antibody (ab122426)

    Lane 1 : Marker
    Lane 2 : HEK293T cell lysate - vector only transfected
    Lane 3 : HEK293T cell lysate - overexpressing C10orf53 with a C-terminal myc-DDK tag (~3.1kDa)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testis tissue labelling C10orf53 with ab122426. Staining shows moderate cytoplasmic positivity in subset of cells in seminiferous ducts.

Anti-C10orf53 antibody (ab122426)参考文献

ab122426 has not yet been referenced specifically in any publications.

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