antigen sequence: MPKNAVVILR YGPYSAAGLP VEHHTFRLQG LQAVLAIDGH EVILEKIEDW NVVELMVNEE VIFHCNIKDL EF, corresponding to N terminal amino acids 1-72 of Human C10orf53
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide Constituents: 59% PBS, 40% Glycerol
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Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/100 - 1/250.
Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Belongs to the UPF0728 family.
Information by UniProt
UPF0728 protein C10orf53 antibody
Immunocytochemistry/ Immunofluorescence - Anti-C10orf53 antibody (ab122426)
Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus but not nucleoli and cytoplasm. Recommended concentration of ab122426 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Western blot - Anti-C10orf53 antibody (ab122426)
Lane 1 : Lanes 2 - 3 : Anti-C10orf53 antibody (ab122426) Lane 1 : Marker Lane 2 : HEK293T cell lysate - vector only transfected Lane 3 : HEK293T cell lysate - overexpressing C10orf53 with a C-terminal myc-DDK tag (~3.1kDa)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C10orf53 antibody (ab122426)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testis tissue labelling C10orf53 with ab122426. Staining shows moderate cytoplasmic positivity in subset of cells in seminiferous ducts.
has not yet been referenced specifically in any publications.
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