Anti-c-Rel (phospho S503)抗体(ab30624)

概述

  • 产品名称Anti-c-Rel (phospho S503)抗体
    参阅全部 c-Rel 一抗
  • 描述
    兔多克隆抗体to c-Rel (phospho S503)
  • 特异性ab30624 recognises endogenous levels of c-Rel only when phosphorylated at Serine 503.
  • 经测试应用适用于: WB, IHC-P, ELISAmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic phosphopeptide derived from human c-Rel around the phosphorylation site of Serine 503.

  • 阳性对照
    • Breast carcinoma, MDA-MB-435 cells

性能

应用

Our Abpromise guarantee covers the use of ab30624 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Predicted molecular weight: 69 kDa.
IHC-P Use at an assay dependent concentration.
ELISA 1/1000.

靶标

  • 功能Proto-oncogene that may play a role in differentiation and lymphopoiesis. NF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. The NF-kappa-B heterodimer RELA/p65-c-Rel is a transcriptional activator.
  • 序列相似性Contains 1 RHD (Rel-like) domain.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Avian reticuloendotheliosis antibody
    • C REL antibody
    • C Rel protein antibody
    • c Rel proto oncogene protein antibody
    • Oncogene REL antibody
    • Oncogene REL avian reticuloendotheliosis antibody
    • Proto-oncogene c-Rel antibody
    • REL antibody
    • REL_HUMAN antibody
    • v rel avian reticuloendotheliosis viral oncogene homolog antibody
    • v rel reticuloendotheliosis viral oncogene homolog antibody
    • V rel reticuloendotheliosis viral oncogene homolog (avian) antibody
    see all

Anti-c-Rel (phospho S503) antibody 图像

  • All lanes : Anti-c-Rel (phospho S503) antibody (ab30624) at 1/500 dilution

    Lane 1 : extracts from MDA-MB-435 cells (5-30ug).
    Lane 2 : extracts from MDA-MB-435 cells (5-30ug), preincubated with synthesized peptide (negative control).


    Predicted band size : 69 kDa
    Observed band size : 60 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemical analysis of paraffin embedded breast carcinoma tissue sections, using 1/50 c-Rel (Phospho- Ser503) Antibody (ab30624). Left: untreated sample; Right: sample preincubated with synthesized phosphopeptide (negative control).

Anti-c-Rel (phospho S503) antibody (ab30624)参考文献

ab30624 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab30624.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"