Anti-c-Rel (phospho S503)抗体(ab30624)
Key features and details
- Rabbit polyclonal to c-Rel (phospho S503)
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-c-Rel (phospho S503)抗体
参阅全部 c-Rel 一抗 -
描述
兔多克隆抗体to c-Rel (phospho S503) -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide corresponding to Human c-Rel (phospho S503).
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阳性对照
- Breast carcinoma, MDA-MB-435 cells
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab30624于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/500 - 1/1000. Predicted molecular weight: 69 kDa.
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IHC-P |
Use at an assay dependent concentration.
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说明 |
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WB
1/500 - 1/1000. Predicted molecular weight: 69 kDa. |
IHC-P
Use at an assay dependent concentration. |
靶标
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功能
Proto-oncogene that may play a role in differentiation and lymphopoiesis. NF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. The NF-kappa-B heterodimer RELA/p65-c-Rel is a transcriptional activator. -
序列相似性
Contains 1 RHD (Rel-like) domain. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 5966 Human
- Omim: 164910 Human
- SwissProt: Q04864 Human
- Unigene: 631886 Human
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别名
- Avian reticuloendotheliosis antibody
- C REL antibody
- C Rel protein antibody
see all
图片
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All lanes : Anti-c-Rel (phospho S503) antibody (ab30624) at 1/500 dilution
Lane 1 : extracts from
MDA-MB-435 cells (5-30ug).
Lane 2 : extracts from
MDA-MB-435 cells (5-30ug), preincubated with synthesized peptide (negative control).
Predicted band size: 69 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted? -
Immunohistochemical analysis of paraffin embedded breast carcinoma tissue sections, using 1/50 c-Rel (Phospho- Ser503) Antibody (ab30624). Left: untreated sample; Right: sample preincubated with synthesized phosphopeptide (negative control).
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab30624 被引用在 1 文献中.
- Xu X et al. Phosphorylation of NF-?Bp65 drives inflammation-mediated hepatocellular carcinogenesis and is a novel therapeutic target. J Exp Clin Cancer Res 40:253 (2021). PubMed: 34380537