重组
RabMAb

Anti-C Reactive蛋白抗体[Y284] (ab32412)

概述

  • 产品名称
    Anti-C Reactive蛋白抗体[Y284]
    参阅全部 C Reactive Protein 一抗
  • 描述
    兔单克隆抗体[Y284] to C Reactive蛋白
  • 宿主
    Rabbit
  • 特异性
    ab32412 recognises C Reactive Protein. This antibody is predicted to detect the splice isoform 2.
  • 经测试应用
    适用于: WB, IP, IHC-Pmore details
    不适用于: ICC/IF
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide within Human C Reactive Protein aa 200 to the C-terminus (C terminal). The exact sequence is proprietary.
    (Peptide available as ab216758)

  • 表位
    ab32412 reacts with an epitope located in the C terminal region of C Reactive Protein.
  • 阳性对照
    • WB: Human serum and plasma lysates. IHC-P: Normal human liver and skeletal muscle tissues. IP: Human serum lysate.
  • 常规说明

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

性能

应用

Our Abpromise guarantee covers the use of ab32412 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/2000. Predicted molecular weight: 25 kDa.Can be blocked with C Reactive Protein peptide (ab216758).
IP 1/30 - 1/50.
IHC-P 1/100 - 1/250.
  • 应用说明
    Is unsuitable for ICC/IF.
  • 靶标

    • 功能
      Displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells.
    • 组织特异性
      Found in plasma.
    • 序列相似性
      Belongs to the pentaxin family.
      Contains 1 pentaxin domain.
    • 细胞定位
      Secreted.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Pentraxin 1, short antibody
      • C reactive protein antibody
      • C reactive protein pentraxin related antibody
      • C-reactive protein(1-205) antibody
      • CRP antibody
      • CRP_HUMAN antibody
      • MGC88244 antibody
      • Pentraxin 1 antibody
      • PTX 1 antibody
      • PTX1 antibody
      see all

    图片

    • All lanes : Anti-C Reactive Protein antibody [Y284] (ab32412) at 1/2000 dilution (purified)

      Lane 1 : Human serum
      Lane 2 : Human plasma

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG at 1/50000 dilution

      Predicted band size: 25 kDa
      Observed band size: 27 kDa (why is the actual band size different from the predicted?)



      Blocking and dilution buffer: 5% NFDM/TBST.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue labelling C Reactive Protein with purified ab32412 at 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skeletal muscle tissue labelling C Reactive Protein with purified ab32412 at 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

    • ab32412 (purified) at 1/30 immunoprecipitating C Reactive Protein in HepG2 whole cell lysate.

      Lane 1 (input): HepG2 whole cell lysate (10µg)

      Lane 2 (+): ab32412 + HepG2 whole cell lysate.

      Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab32412 in HepG2 whole cell lysate.

      For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).

      Blocking buffer and concentration: 5% NFDM/TBST.

      Diluting buffer and concentration: 5% NFDM /TBST.

    • Anti-C Reactive Protein antibody [Y284] (ab32412) at 1/1000 dilution (unpurified) + Human serum

      Predicted band size: 25 kDa
      Observed band size: 27 kDa (why is the actual band size different from the predicted?)

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded) analysis of human normal liver tissue labelling C Reactive Protein using unpurified ab32412 at a dilution of 1/100.

    文献

    This product has been referenced in:
    • Hanna M  et al. Association between physical activity and the expression of mediators of inflammation in normal breast tissue among premenopausal and postmenopausal women. Cytokine 102:151-160 (2018). Read more (PubMed: 29102166) »
    • Hanna M  et al. Association between local inflammation and breast tissue age-related lobular involution among premenopausal and postmenopausal breast cancer patients. PLoS One 12:e0183579 (2017). Read more (PubMed: 28846716) »

    See all 19 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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