Anti-C Reactive蛋白抗体[C6] (ab8278)
Key features and details
- Mouse monoclonal [C6] to C Reactive Protein
- Suitable for: Sandwich ELISA
- Reacts with: Human
- Isotype: IgG2a
Related conjugates and formulations
概述
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产品名称
Anti-C Reactive蛋白抗体[C6]
参阅全部 C Reactive Protein 一抗 -
描述
小鼠单克隆抗体[C6] to C Reactive蛋白 -
宿主
Mouse -
经测试应用
适用于: Sandwich ELISAmore details -
种属反应性
与反应: Human -
免疫原
Full length native protein (purified) corresponding to Human C Reactive Protein.
Database link: P02741 -
常规说明
Specific antibody titer in ELISA is > 10 to the power of -6The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.40
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
C6 -
骨髓瘤
Sp2/0 -
同种型
IgG2a -
轻链类型
unknown -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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sELISA pair antibody
应用
应用 | Ab评论 | 说明 |
---|---|---|
Sandwich ELISA |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Mouse monoclonal [C2] to C Reactive Protein (ab136176). Recommended pairs for sandwich immunoassay
(capture - detection):C2 - C6 C5 - C6C7 - C6. |
说明 |
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Sandwich ELISA
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Mouse monoclonal [C2] to C Reactive Protein (ab136176). Recommended pairs for sandwich immunoassay (capture - detection):C2 - C6C5 - C6 C7 - C6. |
靶标
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功能
Displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells. -
组织特异性
Found in plasma. -
序列相似性
Belongs to the pentaxin family.
Contains 1 pentaxin domain. -
细胞定位
Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 1401 Human
- Omim: 123260 Human
- SwissProt: P02741 Human
- Unigene: 709456 Human
- Unigene: 76452 Human
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别名
- Pentraxin 1, short antibody
- C reactive protein antibody
- C reactive protein pentraxin related antibody
see all
图片
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Calibration curve using ab8277 [C2] (biotinylated) and ab8278 [C6] (labelled with stable Eu3+ chelate) in a sandwich ELISA. Mixture of antibodies and antigen (10
µ l sample) were incubated for 10 minutes at R.T. in streptavidin coated plates. -
Sensitivity to EDTA. Serum samples with (dark blue) and w/o (light blue) Ca2+ ions were tested by two two-site combinations of anti-CRP MAbs. Ab10028 [C4] is sensitive to the conformational changes of CRP molecule and thus the assay gives no signal in the presence of EDTA. Sandwich immunoassay utilizing ab8277 [C2] and ab8278 [C6] recognizes the antigen with the same efficiency in the presence and in the absence of EDTA.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (5)
ab8278 被引用在 5 文献中.
- He G et al. Mitigating hook effect in one-step quantitative sandwich lateral flow assay by timed conjugate release. Talanta 240:123157 (2022). PubMed: 34968809
- Park SB & Shin JH Pressed Lateral Flow Assay Strips for Flow Delay-Induced Signal Enhancement in Lateral Flow Assay Strips. Biochip J 16:480-489 (2022). PubMed: 36320437
- Ouyang M & Di Carlo D Nanoplasmonic swarm biosensing using single nanoparticle colorimetry. Biosens Bioelectron 132:162-170 (2019). PubMed: 30875628
- Zong C et al. Metal-enhanced chemiluminescence detection of C-reaction protein based on silver nanoparticle hybrid probes. Talanta 199:164-169 (2019). PubMed: 30952241
- Cheah IK et al. Administration of Pure Ergothioneine to Healthy Human Subjects: Uptake, Metabolism, and Effects on Biomarkers of Oxidative Damage and Inflammation. Antioxid Redox Signal 26:193-206 (2017). PubMed: 27488221