Anti-C Reactive Protein抗体- Aminoterminal end (ab65842)

概述

  • 产品名称Anti-C Reactive Protein抗体- Aminoterminal end
    参阅全部 C Reactive Protein 一抗
  • 描述
    兔多克隆抗体to C Reactive Protein - Aminoterminal end
  • 经测试应用适用于: WB, IHC-Frmore details
  • 种属反应性
    与反应: Mouse, Rat, Human, Pig
  • 免疫原

    A synthetic peptide corresponding to a sequence at the N-terminal of human C Reactive Protein identical to the related rat and mouse sequence.

  • 阳性对照
    • Rat liver tissue lysate and mouse lung tissue.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab65842 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.1 - 0.5 µg/ml. Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa).
IHC-Fr Use at an assay dependent concentration.

靶标

Anti-C Reactive Protein antibody - Aminoterminal end 图像

  • ab65842 staining C Reactive Protein in mouse lung tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde, permeabilized with 0.5% Triton X-100 and blocked for 5 minutes at 22°C. Samples were incubated with primary antibody (1/500 in 1X PBS) for 17 hours at 4°C. A Biotin-conjugated horse anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.

    See Abreview

  • Anti-C Reactive Protein antibody - Aminoterminal end (ab65842) at 2 µg/ml + rat liver tissue lysate at 30 µg

    Predicted band size : 25 kDa
    Observed band size : 25 kDa

Anti-C Reactive Protein antibody - Aminoterminal end (ab65842)参考文献

ab65842 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Frozen sections)
Blocking step ScyTek Super Block as blocking agent for 5 minute(s) · Concentration: 100% · Temperature: 22°C
Sample Mouse Tissue sections (Lung)
Specification Lung
Permeabilization Yes - 0.5% Triton X-100
Fixative Paraformaldehyde
Username

Mrs. Aleksandra Kotlyarova

Verified customer

提交于 Oct 29 2014

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Pig Tissue sections (Pig Heart Tissue)
Specification Pig Heart Tissue
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 10mM Sodium Citrate
Permeabilization No
Blocking step H2O2 containing sodium azide as blocking agent for 5 minute(s) · Concentration: 0.03%
Username

Tim Thayer

Verified customer

提交于 Jul 28 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"