概述

  • 产品名称Anti-C肽抗体
    参阅全部 C Peptide 一抗
  • 描述
    兔多克隆抗体to C肽
  • 经测试应用适用于: RIA, IHC-Fr, IHC-P, ELISA, ICC, WB, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide corresponding to Human C Peptide aa 57-87 conjugated to Bovine thyroglobulin.
    Sequence:

    EAEDLQVGQVELGGGPGAGSLQPLALEGSLQ


    (Peptide available as ab93903)

  • 常规说明


    C Peptide is part of the molecule of Proinsulin, that consists of three parts: C Peptide and two long strands of amino acids (called the alpha and beta chains) that later become linked together to form the insulin molecule. From every molecule of proinsulin, one molecule of insulin plus one molecule of C Peptide are produced. C peptide is released into the blood stream in equal amounts to insulin. A test of C peptide levels will show how much insulin the body is making. Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: None
    Constituents: Whole Serum
  • 纯度Whole antiserum
  • Primary antibody说明C Peptide is part of the molecule of Proinsulin, that consists of three parts: C Peptide and two long strands of amino acids (called the alpha and beta chains) that later become linked together to form the insulin molecule. From every molecule of proinsulin, one molecule of insulin plus one molecule of C Peptide are produced. C peptide is released into the blood stream in equal amounts to insulin. A test of C peptide levels will show how much insulin the body is making. Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab14181 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
RIA 1/2000.
IHC-Fr 1/100.
IHC-P 1/2000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ELISA 1/8000.
ICC 1/100 - 1/200.
WB 1/100. Can be blocked with Human C Peptide peptide (ab93903). The detection of a C-peptide in Western Blot is relatively difficult, because the peptide is only 5kDa big.
ICC/IF Use at an assay dependent concentration.

靶标

  • 功能Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.
  • 疾病相关Defects in INS are the cause of familial hyperproinsulinemia (FHPRI) [MIM:176730].
    Defects in INS are a cause of diabetes mellitus insulin-dependent type 2 (IDDM2) [MIM:125852]. IDDM2 is a multifactorial disorder of glucose homeostasis that is characterized by susceptibility to ketoacidosis in the absence of insulin therapy. Clinical fetaures are polydipsia, polyphagia and polyuria which result from hyperglycemia-induced osmotic diuresis and secondary thirst. These derangements result in long-term complications that affect the eyes, kidneys, nerves, and blood vessels.
    Defects in INS are a cause of diabetes mellitus permanent neonatal (PNDM) [MIM:606176]. PNDM is a rare form of diabetes distinct from childhood-onset autoimmune diabetes mellitus type 1. It is characterized by insulin-requiring hyperglycemia that is diagnosed within the first months of life. Permanent neonatal diabetes requires lifelong therapy.
    Defects in INS are a cause of maturity-onset diabetes of the young type 10 (MODY10) [MIM:613370]. MODY10 is a form of diabetes that is characterized by an autosomal dominant mode of inheritance, onset in childhood or early adulthood (usually before 25 years of age), a primary defect in insulin secretion and frequent insulin-independence at the beginning of the disease.
  • 序列相似性Belongs to the insulin family.
  • 细胞定位Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • IDDM 2 antibody
    • IDDM2 antibody
    • ILPR antibody
    • ins antibody
    • INS_HUMAN antibody
    • Insulin A chain antibody
    • IRDN antibody
    • MODY10 antibody
    • Proinsulin antibody
    see all

Anti-C Peptide antibody 图像

  • Ab14181 staining human normal pancreas. Staining is localized to the cytoplasm.
    Left panel: with primary antibody diluted 1:2000. Right panel: isotype control.
    Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS), then incubated with primary antibody for 20 minutes, and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

Anti-C Peptide antibody (ab14181)参考文献

This product has been referenced in:
  • Figliolini F  et al. Isolation, characterization and potential role in beta cell-endothelium cross-talk of extracellular vesicles released from human pancreatic islets. PLoS One 9:e102521 (2014). WB ; Human . Read more (PubMed: 25028931) »
  • Reebye V  et al. A Short-activating RNA Oligonucleotide Targeting the Islet ß-cell Transcriptional Factor MafA in CD34(+) Cells. Mol Ther Nucleic Acids 2:e97 (2013). Read more (PubMed: 23736775) »

See all 6 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 10 mm sodium citrate PH6
Sample Human Tissue sections (pancreas)
Specification pancreas
Permeabilization No
Fixative Formaldehyde
Username

Abcam user community

Verified customer

提交于 Jan 15 2015

As requested, I have issued a free of charge replacement for ab14181 (other lot)on the order number *****.

To check the status of the order please contact our Customer Service team and reference this number.

Please note that this free...

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Thank you for those precisions.

I have now found the order placed by ***.
We have in stock a different lot of ab14181, would you agree to receive a replacement vial of this lot?
I just saw that an order has been placed today for this a...

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I am sorry but I cannot find any order matching the PO *****. Imanaged however tofind the order placed in August 2009 corresponding to the lot 739252.

Could you please check with your purchasing department if the order was placed directly to A...

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Thank you for contacting us.

I am sorry to hear you are experiencing difficulties with the anti-C peptide ab14181. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

...

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Thank you for that information.

Small molecular weight proteins can be hindered from moving out of the gel and into the nitrocellulose membrane by SDS. Therefore, I would recommend removing the SDS from the transfer buffer and then seeing that...

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Thank you for your reply.

I just had one further question, what type of transfer buffer were you using and how long do you transfer for (and what voltage/current)? I ask, as since this is a very small protein (5kDa), the transfer conditions th...

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Thank you for contacting Abcam.

The antibody is covered under our Abpromise for six months and is guaranteed to work in RIA, IHC-Fr, IHC-P, WB, ICC/IF on mouse and human samples. If we cannot resolve the issue you are having with the antibody ...

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The ab8297 and ab85715 has been added to the order 1075844.

Thank you very muchfor your cooperation in this case.

Could you let me know which antibody you are interested in to get we have following antibodies in catalogue;

ab85715 and ab95256 are tested in ICC/IF on human cells

ab115199 and ab8297 are also tested in ICC/IF on human cells.

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