Anti-c-Myc (phospho T358)抗体(ab30642)

概述

  • 产品名称Anti-c-Myc (phospho T358)抗体
    参阅全部 c-Myc 一抗
  • 描述
    兔多克隆抗体to c-Myc (phospho T358)
  • 特异性ab30642 detects endogenous levels of Myc only when phosphorylated at Threonine 358.
  • 经测试应用适用于: IHC-P, ELISA, IPmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic phosphopeptide derived from human c-Myc around the phosphorylation site of Threonine 358.

  • 阳性对照
    • Breast carcinoma.

性能

应用

Our Abpromise guarantee covers the use of ab30642 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use at an assay dependent concentration.
ELISA 1/1000.
IP Use at an assay dependent concentration.

靶标

  • 功能Participates in the regulation of gene transcription. Binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3'. Seems to activate the transcription of growth-related genes.
  • 疾病相关Note=Overexpression of MYC is implicated in the etiology of a variety of hematopoietic tumors.
    Note=A chromosomal aberration involving MYC may be a cause of a form of B-cell chronic lymphocytic leukemia. Translocation t(8;12)(q24;q22) with BTG1.
    Defects in MYC are a cause of Burkitt lymphoma (BL) [MIM:113970]. A form of undifferentiated malignant lymphoma commonly manifested as a large osteolytic lesion in the jaw or as an abdominal mass. Note=Chromosomal aberrations involving MYC are usually found in Burkitt lymphoma. Translocations t(8;14), t(8;22) or t(2;8) which juxtapose MYC to one of the heavy or light chain immunoglobulin gene loci.
  • 序列相似性Contains 1 basic helix-loop-helix (bHLH) domain.
  • 翻译后修饰Phosphorylated by PRKDC. Phosphorylation at Thr-58 and Ser-62 by GSK3 is required for ubiquitination and degradation by the proteasome. Phosphorylation at Ser-329 by PIM2 leads to the stabilization of MYC (By similarity). Phosphorylation at Ser-62 by CDK2 prevents Ras-induced senescence.
    Ubiquitinated by the SCF(FBXW7) complex when phosphorylated at Thr-58 and Ser-62, leading to its degradation by the proteasome. In the nucleoplasm, ubiquitination is counteracted by USP28, which interacts with isoform 1 of FBXW7 (FBW7alpha), leading to its deubiquitination and preventing degradation. In the nucleolus, however, ubiquitination is not counteracted by USP28, due to the lack of interaction between isoform 4 of FBXW7 (FBW7gamma) and USP28, explaining the selective MYC degradation in the nucleolus. Also polyubiquitinated by the DCX(TRUSS) complex.
  • 细胞定位Nucleus > nucleoplasm. Nucleus > nucleolus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Avian myelocytomatosis viral oncogene homolog antibody
    • bHLHe39 antibody
    • c Myc antibody
    • Class E basic helix-loop-helix protein 39 antibody
    • MRTL antibody
    • Myc antibody
    • Myc protein antibody
    • Myc proto oncogene protein antibody
    • Myc proto-oncogene protein antibody
    • myc related translation/localization regulatory factor antibody
    • MYC_HUMAN antibody
    • Myc2 antibody
    • MYCC antibody
    • Niard antibody
    • Nird antibody
    • Proto-oncogene c-Myc antibody
    • Transcription factor p64 antibody
    • v myc avian myelocytomatosis viral oncogene homolog antibody
    • v myc myelocytomatosis viral oncogene homolog antibody
    see all

Anti-c-Myc (phospho T358) antibody 图像

  • Immunohistochemical analysis of paraffin-embedded breast carcinoma. Left: Using Phospho-Myc(Thr358) Antibody(ab30642); Right: The same antibody preincubated with synthesized phosphopeptide. Suggested dilution: 1:50 - 1:100

Anti-c-Myc (phospho T358) antibody (ab30642)参考文献

ab30642 has not yet been referenced specifically in any publications.

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