Anti-c-Myc抗体[9E10] (Phycoerythrin) (ab72468)

概述

  • 产品名称Anti-c-Myc抗体[9E10] (Phycoerythrin)
    参阅全部 c-Myc 一抗
  • 描述
    小鼠单克隆抗体[9E10] to c-Myc (Phycoerythrin)
  • 偶联物Phycoerythrin. Ex: 488nm, Em: 575nm
  • 特异性Recognizes the 9E10 epitope (EQKLISEEDL sequence) derived from the human c-myc protein.
  • 经测试应用适用于: Flow Cytmore details
  • 种属反应性
    与反应: Human
    预测可用于: Chimpanzee, Marmoset (common)
  • 免疫原

    Synthetic peptide within Human c-Myc aa 408-432 conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Sequence:

    AEEQKLISEEDLLRKRREQLKHKLE

  • 常规说明

    Conjugate = SureLight® R-Phycoerythrin.

    We recommend that the investigator determine the appropriate working concentration for their specific application.

    Excitation max. lambda: 565>498 nm Emission max. lambda: 578 nm F/P 1:1.

性能

应用

Our Abpromise guarantee covers the use of ab72468 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use at an assay dependent concentration.

Visible absorption maxima 565>540>498

Emission maximum 578





ab91357-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

靶标

  • 功能Participates in the regulation of gene transcription. Binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3'. Seems to activate the transcription of growth-related genes.
  • 疾病相关Note=Overexpression of MYC is implicated in the etiology of a variety of hematopoietic tumors.
    Note=A chromosomal aberration involving MYC may be a cause of a form of B-cell chronic lymphocytic leukemia. Translocation t(8;12)(q24;q22) with BTG1.
    Defects in MYC are a cause of Burkitt lymphoma (BL) [MIM:113970]. A form of undifferentiated malignant lymphoma commonly manifested as a large osteolytic lesion in the jaw or as an abdominal mass. Note=Chromosomal aberrations involving MYC are usually found in Burkitt lymphoma. Translocations t(8;14), t(8;22) or t(2;8) which juxtapose MYC to one of the heavy or light chain immunoglobulin gene loci.
  • 序列相似性Contains 1 basic helix-loop-helix (bHLH) domain.
  • 翻译后修饰Phosphorylated by PRKDC. Phosphorylation at Thr-58 and Ser-62 by GSK3 is required for ubiquitination and degradation by the proteasome.
    Ubiquitinated by the SCF(FBXW7) complex when phosphorylated at Thr-58 and Ser-62, leading to its degradation by the proteasome. In the nucleoplasm, ubiquitination is counteracted by USP28, which interacts with isoform 1 of FBXW7 (FBW7alpha), leading to its deubiquitination and preventing degradation. In the nucleolus, however, ubiquitination is not counteracted by USP28, due to the lack of interaction between isoform 4 of FBXW7 (FBW7gamma) and USP28, explaining the selective MYC degradation in the nucleolus. Also polyubiquitinated by the DCX(TRUSS) complex.
  • 细胞定位Nucleus > nucleoplasm. Nucleus > nucleolus.
  • Information by UniProt
  • 数据库链接
  • 形式c-Myc is also expressed in the cytoplasm.
  • 别名
    • AU016757 antibody
    • Avian myelocytomatosis viral oncogene homolog antibody
    • bHLHe39 antibody
    • c Myc antibody
    • Class E basic helix-loop-helix protein 39 antibody
    • MRTL antibody
    • Myc antibody
    • Myc protein antibody
    • Myc proto oncogene protein antibody
    • Myc proto-oncogene protein antibody
    • myc-related translation/localization regulatory factor antibody
    • MYC_HUMAN antibody
    • Myc2 antibody
    • MYCC antibody
    • Myelocytomatosis oncogene antibody
    • Niard antibody
    • Nird antibody
    • Oncogene Myc antibody
    • OTTHUMP00000158589 antibody
    • Proto-oncogene c-Myc antibody
    • Protooncogene homologous to myelocytomatosis virus antibody
    • RNCMYC antibody
    • Transcription factor p64 antibody
    • Transcriptional regulator Myc-A antibody
    • V-Myc avian myelocytomatosis viral oncogene homolog antibody
    • v-myc myelocytomatosis viral oncogene homolog (avian) antibody
    see all

Anti-c-Myc antibody [9E10] (Phycoerythrin) 图像

  • Fluorescence excitation and emission spectra of R-phycoerythrin in 100 mM sodium phosphate (pH 7.2) + 1mM EDTA and 1 mM sodium azide. Emission scan was taken with excitation at 498 nm. Excitation scan was taken with emission t 575 nm. Scans were normalized to equalize peak heights.

Anti-c-Myc antibody [9E10] (Phycoerythrin) (ab72468)参考文献

ab72468 has not yet been referenced specifically in any publications.

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