概述

  • 产品名称Anti-c-Myc抗体[3G30] (FITC)
    参阅全部 c-Myc 一抗
  • 描述
    小鼠单克隆抗体[3G30] to c-Myc (FITC)
  • 偶联物FITC. Ex: 493nm, Em: 528nm
  • 特异性This antibody recognizes proteins tagged with c-myc at either the C-terminus or the N-terminus as well as Human c-Myc protein.
  • 经测试应用适用于: WB, IP, ELISA, ICC/IF, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide corresponding to amino acids 408-439 of human c-Myc.

  • 表位This antibody recognizes an epitope encoded by the amino acid sequence EQKLISEEDL, corresponding to aa 410–419 in the leucine zipper region of human c-myc.
  • 常规说明

    Many expression plasmids encoding the sequence EQKLISEEDL are available for producing c-myc tagged fusion proteins.

    Fluorochrome/Antibody ratio: 0.98.

性能

应用

Our Abpromise guarantee covers the use of ab69258 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent concentration. Detects a band of approximately 65 kDa (predicted molecular weight: 49 kDa).

Detects a band of approximately 65 kDa in reducing SDS-PAGE.

IP Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
ICC/IF Use a concentration of 2 µg/ml.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

靶标

  • 功能Participates in the regulation of gene transcription. Binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3'. Seems to activate the transcription of growth-related genes.
  • 疾病相关Note=Overexpression of MYC is implicated in the etiology of a variety of hematopoietic tumors.
    Note=A chromosomal aberration involving MYC may be a cause of a form of B-cell chronic lymphocytic leukemia. Translocation t(8;12)(q24;q22) with BTG1.
    Defects in MYC are a cause of Burkitt lymphoma (BL) [MIM:113970]. A form of undifferentiated malignant lymphoma commonly manifested as a large osteolytic lesion in the jaw or as an abdominal mass. Note=Chromosomal aberrations involving MYC are usually found in Burkitt lymphoma. Translocations t(8;14), t(8;22) or t(2;8) which juxtapose MYC to one of the heavy or light chain immunoglobulin gene loci.
  • 序列相似性Contains 1 basic helix-loop-helix (bHLH) domain.
  • 翻译后修饰Phosphorylated by PRKDC. Phosphorylation at Thr-58 and Ser-62 by GSK3 is required for ubiquitination and degradation by the proteasome.
    Ubiquitinated by the SCF(FBXW7) complex when phosphorylated at Thr-58 and Ser-62, leading to its degradation by the proteasome. In the nucleoplasm, ubiquitination is counteracted by USP28, which interacts with isoform 1 of FBXW7 (FBW7alpha), leading to its deubiquitination and preventing degradation. In the nucleolus, however, ubiquitination is not counteracted by USP28, due to the lack of interaction between isoform 4 of FBXW7 (FBW7gamma) and USP28, explaining the selective MYC degradation in the nucleolus. Also polyubiquitinated by the DCX(TRUSS) complex.
  • 细胞定位Nucleus > nucleoplasm. Nucleus > nucleolus.
  • Information by UniProt
  • 数据库链接
  • 形式c-Myc is also expressed in the cytoplasm.
  • 别名
    • AU016757 antibody
    • Avian myelocytomatosis viral oncogene homolog antibody
    • bHLHe39 antibody
    • c Myc antibody
    • Class E basic helix-loop-helix protein 39 antibody
    • MRTL antibody
    • Myc antibody
    • Myc protein antibody
    • Myc proto oncogene protein antibody
    • Myc proto-oncogene protein antibody
    • myc-related translation/localization regulatory factor antibody
    • MYC_HUMAN antibody
    • Myc2 antibody
    • MYCC antibody
    • Myelocytomatosis oncogene antibody
    • Niard antibody
    • Nird antibody
    • Oncogene Myc antibody
    • OTTHUMP00000158589 antibody
    • Proto-oncogene c-Myc antibody
    • Protooncogene homologous to myelocytomatosis virus antibody
    • RNCMYC antibody
    • Transcription factor p64 antibody
    • Transcriptional regulator Myc-A antibody
    • V-Myc avian myelocytomatosis viral oncogene homolog antibody
    • v-myc myelocytomatosis viral oncogene homolog (avian) antibody
    see all

Anti-c-Myc antibody [3G30] (FITC) (ab69258)参考文献

This product has been referenced in:
  • Noach-Hirsh M  et al. Integrated Microfluidics for Protein Modification Discovery. Mol Cell Proteomics 14:2824-32 (2015). Read more (PubMed: 26276765) »
  • Singh H  et al. MitoBK(Ca) is encoded by the Kcnma1 gene, and a splicing sequence defines its mitochondrial location. Proc Natl Acad Sci U S A 110:10836-41 (2013). Read more (PubMed: 23754429) »

See all 2 Publications for this product

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