纯化说明ab111339 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
功能Transcriptional activator; DNA-binding protein that specifically recognize the sequence 5'-YAAC[GT]G-3'. Plays an important role in the control of proliferation and differentiation of hematopoietic progenitor cells.
序列相似性Contains 3 HTH myb-type DNA-binding domains.
结构域Comprised of 3 domains; an N-terminal DNA-binding domain, a centrally located transcriptional activation domain and a C-terminal domain involved in transcriptional repression.
翻译后修饰Ubiquitinated; mediated by SIAH1 and leading to its subsequent proteasomal degradation. Phosphorylated by NLK on multiple sites, which induces proteasomal degradation.
Immunohistochemistry analysis of paraffin-embedded Human breast carcinoma tissue, using ab111339 at a dilution of 1/50. Left panel shows staining without immunizing peptide right panel shows staining with immunizing peptide.
Anti-c-Myb (phospho S532) antibody (ab111339)参考文献
has not yet been referenced specifically in any publications.