概述

  • 产品名称
    Anti-c-Myb抗体[EPR718(2)]
    参阅全部 c-Myb 一抗
  • 描述
    兔单克隆抗体[EPR718(2)] to c-Myb
  • 经测试应用
    适用于: IHC-P, WBmore details
    不适用于: Flow Cyt,ICC or IP
  • 种属反应性
    与反应: Human
  • 免疫原

    A synthetic peptide corresponding to residues in Human c-Myb.

  • 阳性对照
    • Jurkat and MOLT4 cell lysates; Human colon tissue
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • 存储溶液
    PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • 纯度
    Tissue culture supernatant
  • 克隆
    单克隆
  • 克隆编号
    EPR718(2)
  • 同种型
    IgG
  • 研究领域

相关产品

应用

Our Abpromise guarantee covers the use of ab109127 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. PubMed: 22569827
WB 1/1000 - 1/10000. Predicted molecular weight: 72 kDa.
  • 应用说明
    Is unsuitable for Flow Cyt,ICC or IP.
  • 靶标

    • 功能
      Transcriptional activator; DNA-binding protein that specifically recognize the sequence 5'-YAAC[GT]G-3'. Plays an important role in the control of proliferation and differentiation of hematopoietic progenitor cells.
    • 序列相似性
      Contains 3 HTH myb-type DNA-binding domains.
    • 结构域
      Comprised of 3 domains; an N-terminal DNA-binding domain, a centrally located transcriptional activation domain and a C-terminal domain involved in transcriptional repression.
    • 翻译后修饰
      Ubiquitinated; mediated by SIAH1 and leading to its subsequent proteasomal degradation.
      Phosphorylated by NLK on multiple sites, which induces proteasomal degradation.
    • 细胞定位
      Nucleus.
    • Information by UniProt
    • 数据库链接
    • 别名
      • C myb antibody
      • c myb protein (140 AA) antibody
      • c-myb protein (140 AA) antibody
      • c-myb_CDS antibody
      • c-myb10A_CDS antibody
      • c-myb13A_CDS antibody
      • c-myb14A_CDS antibody
      • c-myb8B_CDS antibody
      • Cmyb antibody
      • efg antibody
      • Myb antibody
      • MYB proto-oncogene transcription factor antibody
      • MYB_HUMAN antibody
      • ONCOGENE AMV antibody
      • Oncogene MYB antibody
      • Proto oncogene c Myb antibody
      • Proto-oncogene c-Myb antibody
      • Transcriptional activator Myb antibody
      • v myb avian myeloblastosis viral oncogene homolog antibody
      • v-myb avian myeloblastosis viral oncogene homolog antibody
      see all

    图片



    • Predicted band size : 72 kDa

      Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
      Lane 2: c-Myb knockout HAP1 whole cell lysate (20 µg)

      Lanes 1 - 2: Merged signal (red and green). Green - ab109127 observed at 75 kDa. Red - loading control, ab9484, observed at 37 kDa.

      ab109127 was shown to specifically react with c-Myb in wild-type HAP1 cells as signal was lost in c-Myb knockout cells. Wild-type and c-Myb knockout samples were subjected to SDS-PAGE. Ab109127 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    • All lanes : Anti-c-Myb antibody [EPR718(2)] (ab109127) at 1/1000 dilution

      Lane 1 : Jurkat cell lysates
      Lane 2 : MOLT4 cell lysates

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 72 kDa

    文献

    This product has been referenced in:

    See all 4 Publications for this product

    客户评价及客户问答

    There are currently no Customer reviews or Questions for ab109127.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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