Anti-c-Myb抗体(ab62824)

概述

  • 产品名称Anti-c-Myb抗体
    参阅全部 c-Myb 一抗
  • 描述
    山羊多克隆抗体to c-Myb
  • 经测试应用适用于: IHC-P, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Chicken, Cow, Dog, Chimpanzee, Zebrafish
  • 免疫原

    Synthetic peptide:

    C-QRHYNDEDPEKEKR

    , corresponding to internal sequence amino acids 281-294 of Human c-Myb.

  • 阳性对照
    • Human Spleen lysate

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab62824 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 3 - 6 µg/ml.
WB Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 72 kDa).

靶标

  • 功能Transcriptional activator; DNA-binding protein that specifically recognize the sequence 5'-YAAC[GT]G-3'. Plays an important role in the control of proliferation and differentiation of hematopoietic progenitor cells.
  • 序列相似性Contains 3 HTH myb-type DNA-binding domains.
  • 结构域Comprised of 3 domains; an N-terminal DNA-binding domain, a centrally located transcriptional activation domain and a C-terminal domain involved in transcriptional repression.
  • 翻译后修饰Ubiquitinated; mediated by SIAH1 and leading to its subsequent proteasomal degradation.
    Phosphorylated by NLK on multiple sites, which induces proteasomal degradation.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • C myb antibody
    • c myb protein (140 AA) antibody
    • c-myb protein (140 AA) antibody
    • c-myb_CDS antibody
    • c-myb10A_CDS antibody
    • c-myb13A_CDS antibody
    • c-myb14A_CDS antibody
    • c-myb8B_CDS antibody
    • Cmyb antibody
    • efg antibody
    • Myb antibody
    • MYB_HUMAN antibody
    • ONCOGENE AMV antibody
    • Oncogene MYB antibody
    • Proto oncogene c Myb antibody
    • Proto-oncogene c-Myb antibody
    • Transcriptional activator Myb antibody
    • v myb avian myeloblastosis viral oncogene homolog antibody
    • v-myb avian myeloblastosis viral oncogene homolog antibody
    see all

Anti-c-Myb antibody 图像

  • Anti-c-Myb antibody (ab62824) at 0.3 µg/ml + Human Spleen lysate in RIPA buffer at 35 µg

    Predicted band size : 72 kDa
    Observed band size : 75 kDa (why is the actual band size different from the predicted?)
  • ab62824 at 4µg/ml staining c-Myb in Human Kidney tissue. Steamed antigen retrieval with EDTA buffer pH 9 was performed, and the sample was stained with HRP.
  • ab62824 at 4µg/ml staining c-Myb in Human Breast tissue. Steamed antigen retrieval with citrate buffer pH 6 was performed, and the sample was stained with HRP.

Anti-c-Myb antibody (ab62824)参考文献

ab62824 has not yet been referenced specifically in any publications.

Product Wall

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab117635.

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Read More
Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (mouse haematopoietic progenitor cell line)
Loading amount 40 µg
Specification mouse haematopoietic progenitor cell line
Gel Running Conditions Reduced Denaturing (7.5% gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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提交于 Jun 06 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"