Anti-c-Myb抗体(ab117635)

概述

  • 产品名称
  • 描述
    兔多克隆抗体to c-Myb
  • 经测试应用
    适用于: WB, ELISA, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse
  • 免疫原

    KLH-conjugated synthetic peptide derived from the N terminal region of Human c-Myb.

  • 阳性对照
    • Human breast or tonisl tissue. HUVEC cell lysate. HeLa cells.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Store undiluted.
  • 存储溶液
    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 50% Glycerol, 0.88% Sodium chloride, 48% PBS
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab117635 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Predicted molecular weight: 72 kDa.
ELISA 1/10000.
IHC-P Use a concentration of 5 µg/ml.
ICC/IF 1/100 - 1/500.

靶标

  • 功能
    Transcriptional activator; DNA-binding protein that specifically recognize the sequence 5'-YAAC[GT]G-3'. Plays an important role in the control of proliferation and differentiation of hematopoietic progenitor cells.
  • 序列相似性
    Contains 3 HTH myb-type DNA-binding domains.
  • 结构域
    Comprised of 3 domains; an N-terminal DNA-binding domain, a centrally located transcriptional activation domain and a C-terminal domain involved in transcriptional repression.
  • 翻译后修饰
    Ubiquitinated; mediated by SIAH1 and leading to its subsequent proteasomal degradation.
    Phosphorylated by NLK on multiple sites, which induces proteasomal degradation.
  • 细胞定位
    Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • C myb antibody
    • c myb protein (140 AA) antibody
    • c-myb protein (140 AA) antibody
    • c-myb_CDS antibody
    • c-myb10A_CDS antibody
    • c-myb13A_CDS antibody
    • c-myb14A_CDS antibody
    • c-myb8B_CDS antibody
    • Cmyb antibody
    • efg antibody
    • Myb antibody
    • MYB proto-oncogene transcription factor antibody
    • MYB_HUMAN antibody
    • ONCOGENE AMV antibody
    • Oncogene MYB antibody
    • Proto oncogene c Myb antibody
    • Proto-oncogene c-Myb antibody
    • Transcriptional activator Myb antibody
    • v myb avian myeloblastosis viral oncogene homolog antibody
    • v-myb avian myeloblastosis viral oncogene homolog antibody
    see all

图片

  • All lanes : Anti-c-Myb antibody (ab117635) at 1/500 dilution

    Lane 1 : HUVEC cell lysate
    Lane 2 : HUVEC cell lysate with synthetic peptide


    Predicted band size : 72 kDa
  • Immunofluorescence analysis of HeLa cells, using 1/100 ab117635.
    The picture on the right is treated with the synthesized peptide.
  • Human Breast, Formalin-Fixed, Paraffin-Embedded tissue, stained with ab117635 at 5µg/ml.
  • Human Tonsil, Formalin-Fixed, Paraffin-Embedded tissue, stained with ab117635 at 5µg/ml.

文献

ab117635 has not yet been referenced specifically in any publications.

客户评价及客户问答

Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Liver from 28 days-old mouse)
Permeabilization
Yes - Triton X-100 0.5%
Specification
Liver from 28 days-old mouse
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Fixative
Paraformaldehyde
Username

Mr. Joan Mir

Verified customer

提交于 Mar 06 2017

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab117635.

To check the status of the order please contact ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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