概述

  • 产品名称Anti-c-Maf抗体
    参阅全部 c-Maf 一抗
  • 描述
    小鼠单克隆抗体to c-Maf
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    GST-tagged recombinant fragment: SCRFKRVQQR HVLESEKNQL LQQVDHLKQE ISRLVRERDA YKEKYEKLVS SGFRENGSSS DNPSSPEFFI TEPTRKLEPS VGYATFWKPQ HRVLTSVFTK , corresponding to amino acids 304-404 of Human c-Maf

  • 阳性对照
    • PC-12 cell lysate

性能

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应用

Our Abpromise guarantee covers the use of ab76817 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 42 kDa.
ELISA Use at an assay dependent dilution.

靶标

  • 功能Acts as a transcriptional activator or repressor. Involved in embryonic lens fiber cell development. Recruits the transcriptional coactivators CREBBP and/or EP300 to crystallin promoters leading to up-regulation of crystallin gene during lens fiber cell differentiation. Activates the expression of IL4 in T helper 2 (Th2) cells. Increases T cell susceptibility to apoptosis by interacting with MYB and decreasing BCL2 expression. Together with PAX6, transactivates strongly the glucagon gene promoter through the G1 element. Activates transcription of the CD13 proximal promoter in endothelial cells. Represses transcription of the CD13 promoter in early stages of myelopoiesis by affecting the ETS1 and MYB cooperative interaction. Involved in the initial chondrocyte terminal differentiation and the disappearance of hypertrophic chondrocytes during endochondral bone development. Binds to the sequence 5'-[GT]G[GC]N[GT]NCTCAGNN-3' in the L7 promoter. Binds to the T-MARE (Maf response element) sites of lens-specific alpha- and beta-crystallin gene promoters. Binds element G1 on the glucagon promoter. Binds an AT-rich region adjacent to the TGC motif (atypical Maf response element) in the CD13 proximal promoter in endothelial cells (By similarity). When overexpressed, represses anti-oxidant reponse element (ARE)-mediated transcription. Involved either as an oncogene or as a tumor suppressor, depending on the cell context. Binds to the ARE sites of detoxifying enzyme gene promoters.
  • 组织特异性Expressed in endothelial cells.
  • 疾病相关Note=A chromosomal aberration involving MAF is found in some forms of multiple myeloma (MM). Translocation t(14;16)(q32.3;q23) with an IgH locus.
    Defects in MAF are the cause of cataract pulverulent juvenile-onset MAF-related (CAPJOM) [MIM:610202]. A form of cataract with nuclear or cortical pulverulent opacities. Pulverulent cataracts are characterized by a dust-like, 'pulverised' appearance of the opacities which can be found in any part of the lens. The phenotype shows significant intra- and interfamilial variation, both in the distribution of the cataract and the degree of opacification. Some patients with cataract pulverulent juvenile-onset can present microcornea and bilateral iris colobomas in addition to cataract.
    Defects in MAF are the cause of cataract congenital cerulean type 4 (CCA4) [MIM:610202]. A cerulean form of congenital cataract. Cerulean cataracts are characterized by peripheral bluish and white opacifications organized in concentric layers with occasional central lesions arranged radially. The opacities are observed in the superficial layers of the fetal nucleus as well as the adult nucleus of the lens. Involvement is usually bilateral. Visual acuity is only mildly reduced in childhood. In adulthood, the opacifications may progress, making lens extraction necessary. Histologically the lesions are described as fusiform cavities between lens fibers which contain a deeply staining granular material. Although the lesions may take on various colors, a dull blue is the most common appearance and is responsible for the designation cerulean cataract.
  • 序列相似性Belongs to the bZIP family. Maf subfamily.
    Contains 1 bZIP domain.
  • 翻译后修饰Ubiquitinated, leading to its degradation by the proteasome. Ubiquitination is triggered by glucocorticoids.
    Phosphorylated by GSK3 and MAPK13 on serine and threonine residues (Probable). The phosphorylation status can serve to either stimulate or inhibit transcription.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AS42 oncogene homolog antibody
    • Avian musculoaponeurotic fibrosarcoma (MAF) protooncogene antibody
    • Avian musculoaponeurotic fibrosarcoma (v maf) antibody
    • c maf proto oncogene antibody
    • cMaf antibody
    • maf antibody
    • MAF_HUMAN antibody
    • MAF2 antibody
    • MGC71685 antibody
    • Proto oncogene c Maf antibody
    • Proto-oncogene c-maf antibody
    • Transcription factor Maf antibody
    • v maf musculoaponeurotic fibrosarcoma oncogene homolog (avian) antibody
    • v maf musculoaponeurotic fibrosarcoma oncogene homolog antibody
    • V-maf musculoaponeurotic fibrosarcoma oncogene homolog antibody
    see all

Anti-c-Maf antibody 图像

  • Anti-c-Maf antibody (ab76817) at 5 µg/ml + immunogen at 0.2 µg

    Secondary
    Goat Anti-Mouse IgG (H&L) HRP-conjugated at 1/5000 dilution

    Predicted band size : 42 kDa
    Observed band size : 40 kDa (why is the actual band size different from the predicted?)
  • Anti-c-Maf antibody (ab76817) at 5 µg/ml + PC-12 cell lysate at 25 µg

    Secondary
    Goat Anti-Mouse IgG (H&L) HRP-conjugated at 1/2500 dilution

    Predicted band size : 42 kDa
    Observed band size : 42 kDa

Anti-c-Maf antibody (ab76817)参考文献

This product has been referenced in:
  • Su W  et al. The p53 transcription factor modulates microglia behavior through microRNA-dependent regulation of c-Maf. J Immunol 192:358-66 (2014). WB . Read more (PubMed: 24319262) »

See 1 Publication for this product

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