Anti-c-Kit (phospho Y703)抗体(ab62154)


  • 产品名称Anti-c-Kit (phospho Y703)抗体
    参阅全部 c-Kit 一抗
  • 描述
    兔多克隆抗体to c-Kit (phospho Y703)
  • 特异性ab62154 detects endogenous levels of c-Kit only when phosphorylated at tyrosine 703 (Human: Tyr703; Mouse: Tyr702).
  • 经测试应用适用于: WB, IHC-P, ELISAmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic phosphopeptide derived from human c-Kit around the phosphorylation site of tyrosine 703 (A-L-YP-K-N).

  • 阳性对照
    • Human breast carcinoma tissue.



Our Abpromise guarantee covers the use of ab62154 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent concentration.
IHC-P 1/50 - 1/100.
ELISA 1/5000.


  • 功能Tyrosine-protein kinase that acts as cell-surface receptor for the cytokine KITLG/SCF and plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. In response to KITLG/SCF binding, KIT can activate several signaling pathways. Phosphorylates PIK3R1, PLCG1, SH2B2/APS and CBL. Activates the AKT1 signaling pathway by phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase. Activated KIT also transmits signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. Promotes activation of STAT family members STAT1, STAT3, STAT5A and STAT5B. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KIT signaling is modulated by protein phosphatases, and by rapid internalization and degradation of the receptor. Activated KIT promotes phosphorylation of the protein phosphatases PTPN6/SHP-1 and PTPRU, and of the transcription factors STAT1, STAT3, STAT5A and STAT5B. Promotes phosphorylation of PIK3R1, CBL, CRK (isoform Crk-II), LYN, MAPK1/ERK2 and/or MAPK3/ERK1, PLCG1, SRC and SHC1.
  • 组织特异性Isoform 1 and isoform 2 are detected in spermatogonia and Leydig cells. Isoform 3 is detected in round spermatids, elongating spermatids and spermatozoa (at protein level). Widely expressed. Detected in the hematopoietic system, the gastrointestinal system, in melanocytes and in germ cells.
  • 疾病相关Piebald trait
    Gastrointestinal stromal tumor
    Testicular germ cell tumor
    Leukemia, acute myelogenous
  • 序列相似性Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
    Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 protein kinase domain.
  • 翻译后修饰Ubiquitinated by SOCS6. KIT is rapidly ubiquitinated after autophosphorylation induced by KITLG/SCF binding, leading to internalization and degradation.
    Autophosphorylated on tyrosine residues. KITLG/SCF binding enhances autophosphorylation. Isoform 1 shows low levels of tyrosine phosphorylation in the absence of added KITLG/SCF (in vitro). Kinase activity is down-regulated by phosphorylation on serine residues by protein kinase C family members. Phosphorylation at Tyr-568 is required for interaction with PTPN11/SHP-2, CRK (isoform Crk-II) and members of the SRC tyrosine-protein kinase family. Phosphorylation at Tyr-570 is required for interaction with PTPN6/SHP-1. Phosphorylation at Tyr-703, Tyr-823 and Tyr-936 is important for interaction with GRB2. Phosphorylation at Tyr-721 is important for interaction with PIK3R1. Phosphorylation at Tyr-823 and Tyr-936 is important for interaction with GRB7.
  • 细胞定位Cell membrane and Cytoplasm. Detected in the cytoplasm of spermatozoa, especially in the equatorial and subacrosomal region of the sperm head.
  • Information by UniProt
  • 数据库链接
  • 别名
    • C Kit antibody
    • c-Kit antibody
    • c-Kit Ligand antibody
    • CD117 antibody
    • Kit antibody
    • Kit Ligand antibody
    • KIT oncogene antibody
    • KIT proto oncogene receptor tyrosine kinase antibody
    • KIT_HUMAN antibody
    • Mast cell growth factor receptor antibody
    • Mast/stem cell growth factor receptor Kit antibody
    • MGF antibody
    • p145 c-kit antibody
    • PBT antibody
    • Piebald trait protein antibody
    • Proto oncogene c Kit antibody
    • Proto oncogene tyrosine protein kinase Kit antibody
    • Proto-oncogene c-Kit antibody
    • SCF Receptor antibody
    • SCFR antibody
    • soluble KIT variant 1 antibody
    • Steel Factor Receptor antibody
    • Stem cell factor receptor antibody
    • tyrosine protein kinase Kit antibody
    • Tyrosine-protein kinase Kit antibody
    • v kit Hardy Zuckerman 4 feline sarcoma viral oncogene homolog antibody
    • v kit Hardy Zuckerman 4 feline sarcoma viral oncogene like protein antibody
    • v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog antibody
    see all

Anti-c-Kit (phospho Y703) antibody 图像

  • Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using c-Kit (phospho Y703) antibody (ab62154) at 1/50 - 1/100 dilution, in the presence (right panel) and absence (left panel) of immunising phosphopeptide.
  • All lanes : Anti-c-Kit (phospho Y703) antibody (ab62154)

    Lane 1 : Colon cancer lysate (human)
    Lane 2 : U2OS lysate (human)
    Lane 3 : Renal cancer cell line (human)

    Swine ati-rabbit HRP at 1/5000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 6 minutes

    This image is courtesy of an Abreview submitted by Antibody Solutions

    See Abreview

Anti-c-Kit (phospho Y703) antibody (ab62154)参考文献

This product has been referenced in:
  • Grewal N  et al. Bicuspid aortic valve: phosphorylation of c-Kit and downstream targets are prognostic for future aortopathy. Eur J Cardiothorac Surg 46:831-9 (2014). Read more (PubMed: 25161185) »

See 1 Publication for this product

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Thank you for your reply.

We do have a few other rabbit polyclonal isotype controls that are at a higher concentration, these are:

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Thank you for your reply.

As this is a rabbit polyclonal antibody, the exact clonality of the antibody cannot be determined. However we do have isotype controls that would work for you, please see the link below:


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Thank you for contacting Abcam.

I have attached the IHC-P protocol that was used to test ab62154 and currently we do not know if any publications that have used this antibody in IHC-P, that does not mean there are not any, just that we do not ...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Colon cancer (tissue lysate), U2OS (cell lysate) a)
Loading amount 20 µg
Specification Colon cancer (tissue lysate), U2OS (cell lysate) a
Gel Running Conditions Reduced Denaturing (Pierce precise protein gel 4-20%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

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提交于 Apr 20 2011