Anti-c-Kit抗体[2B8] (FITC) (ab24870)

概述

  • 产品名称Anti-c-Kit抗体[2B8] (FITC)
    参阅全部 c-Kit 一抗
  • 描述
    大鼠单克隆抗体[2B8] to c-Kit (FITC)
  • 偶联物FITC. Ex: 493nm, Em: 528nm
  • 经测试应用适用于: ICC/IF, Flow Cytmore details
  • 种属反应性
    与反应: Mouse
  • 免疫原

    The details of the immunogen for this antibody are not available.

  • 阳性对照
    • BALB/C bone marrow cells.

应用

Our Abpromise guarantee covers the use of ab24870 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent concentration. PubMed: 22387295
Flow Cyt Use 1µg for 106 cells.

Also suitable for cell homing and hematopoiesis and development studies.
ab136125 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

靶标

  • 功能Tyrosine-protein kinase that acts as cell-surface receptor for the cytokine KITLG/SCF and plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. In response to KITLG/SCF binding, KIT can activate several signaling pathways. Phosphorylates PIK3R1, PLCG1, SH2B2/APS and CBL. Activates the AKT1 signaling pathway by phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase. Activated KIT also transmits signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. Promotes activation of STAT family members STAT1, STAT3, STAT5A and STAT5B. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KIT signaling is modulated by protein phosphatases, and by rapid internalization and degradation of the receptor. Activated KIT promotes phosphorylation of the protein phosphatases PTPN6/SHP-1 and PTPRU, and of the transcription factors STAT1, STAT3, STAT5A and STAT5B. Promotes phosphorylation of PIK3R1, CBL, CRK (isoform Crk-II), LYN, MAPK1/ERK2 and/or MAPK3/ERK1, PLCG1, SRC and SHC1.
  • 组织特异性Isoform 1 and isoform 2 are detected in spermatogonia and Leydig cells. Isoform 3 is detected in round spermatids, elongating spermatids and spermatozoa (at protein level). Widely expressed. Detected in the hematopoietic system, the gastrointestinal system, in melanocytes and in germ cells.
  • 疾病相关Piebald trait
    Gastrointestinal stromal tumor
    Testicular germ cell tumor
    Leukemia, acute myelogenous
  • 序列相似性Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
    Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 protein kinase domain.
  • 翻译后修饰Ubiquitinated by SOCS6. KIT is rapidly ubiquitinated after autophosphorylation induced by KITLG/SCF binding, leading to internalization and degradation.
    Autophosphorylated on tyrosine residues. KITLG/SCF binding enhances autophosphorylation. Isoform 1 shows low levels of tyrosine phosphorylation in the absence of added KITLG/SCF (in vitro). Kinase activity is down-regulated by phosphorylation on serine residues by protein kinase C family members. Phosphorylation at Tyr-568 is required for interaction with PTPN11/SHP-2, CRK (isoform Crk-II) and members of the SRC tyrosine-protein kinase family. Phosphorylation at Tyr-570 is required for interaction with PTPN6/SHP-1. Phosphorylation at Tyr-703, Tyr-823 and Tyr-936 is important for interaction with GRB2. Phosphorylation at Tyr-721 is important for interaction with PIK3R1. Phosphorylation at Tyr-823 and Tyr-936 is important for interaction with GRB7.
  • 细胞定位Cell membrane and Cytoplasm. Detected in the cytoplasm of spermatozoa, especially in the equatorial and subacrosomal region of the sperm head.
  • Information by UniProt
  • 数据库链接
  • 别名
    • C Kit antibody
    • c-Kit antibody
    • c-Kit Ligand antibody
    • CD117 antibody
    • Kit antibody
    • Kit Ligand antibody
    • KIT oncogene antibody
    • KIT proto oncogene receptor tyrosine kinase antibody
    • KIT_HUMAN antibody
    • Mast cell growth factor receptor antibody
    • Mast/stem cell growth factor receptor Kit antibody
    • MGF antibody
    • p145 c-kit antibody
    • PBT antibody
    • Piebald trait protein antibody
    • Proto oncogene c Kit antibody
    • Proto oncogene tyrosine protein kinase Kit antibody
    • Proto-oncogene c-Kit antibody
    • SCF Receptor antibody
    • SCFR antibody
    • soluble KIT variant 1 antibody
    • Steel Factor Receptor antibody
    • Stem cell factor receptor antibody
    • tyrosine protein kinase Kit antibody
    • Tyrosine-protein kinase Kit antibody
    • v kit Hardy Zuckerman 4 feline sarcoma viral oncogene homolog antibody
    • v kit Hardy Zuckerman 4 feline sarcoma viral oncogene like protein antibody
    • v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog antibody
    see all

Anti-c-Kit antibody [2B8] (FITC) 图像

  • ab24870 staining cKit in mouse bone marrow cells by Flow Cytometry. 10 µL primary antibody was added to 50 µL cell suspension in PBS with 1% normal mouse serum (500,000 cells). Gating strategy: mesenchymal stem cells.

    See Abreview

  • Flow Cytometry analysis of BALB/c mouse bone marrow cells labeling c-Kit with ab24870 at 0.01 μg/106 cells dilution. A Rat Anti-Mouse CD45R-PE was used as the secondary antibody. 

Anti-c-Kit antibody [2B8] (FITC) (ab24870)参考文献

This product has been referenced in:
  • Zeng SL  et al. Mesenchymal stem cells abrogate experimental asthma by altering dendritic cell function. Mol Med Rep 12:2511-20 (2015). Read more (PubMed: 25936350) »
  • Gerasimovskaya E  et al. Interplay of macrophages and T cells in the lung vasculature. Am J Physiol Lung Cell Mol Physiol 302:L1014-22 (2012). ICC/IF ; Rat . Read more (PubMed: 22387295) »

See all 3 Publications for this product

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DISCOUNT CODE: ***
Expiration date: November 27th, 2012

I am very pleased to hear you would like to accept our offer and test ab24870 in rat. This code will give you: 1 free primary antibodybefore the expiration date. To redeem this offe...

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The isotype control "Rat IgG2b, kappa (FITC) - Isotype Control" ab136125 would indeed be the most appropriate control to suggest for use with ab24870 in FACS.
I hope this has been helpful. If I can be of any further he...

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Thank you for contacting Abcam.

Based on the protocol for the specific reference on our website that has used ab24870 for ICC/IF (PMID:
22387295 http://www.ncbi.nlm.nih.gov/pubmed/22387295?dopt=Abstract) the researchers incubated with prim...

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Thank you for contacting us.


There have been reports of ab24870, Rat Anti-Mouse CD117/c-kit, showing cross reactivity with Rat; however, we have not verified that through in house testing.

As we have not tested this in house, fo...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Sample Mouse Cell (mouse bone marrow cells)
Specification mouse bone marrow cells
Preparation Cell harvesting/tissue preparation method: fresh isolated
Sample buffer: PBS
Fixation no
Permeabilization No
Gating Strategy mesenchymal stem cells
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提交于 Sep 16 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"