概述

  • 产品名称Anti-c-Jun (phospho S243)抗体
    参阅全部 c-Jun 一抗
  • 描述
    兔多克隆抗体to c-Jun (phospho S243)
  • 特异性c-Jun (Phospho-Ser243) Antibody detects endogenous levels of c-Jun only when phosphorylated at Ser243.
  • 经测试应用适用于: WB, IHC-P, IP, ICC/IF, ELISAmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to Human c-Jun (phospho S243). Immunogen is in the range of aa 210 - 259
    Database link: P05412

  • 阳性对照
    • WB: HeLa cell lysate. IHC-P: human breast carcinoma tissue.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab28846 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 39 kDa (predicted molecular weight: 36 kDa).
IHC-P 1/50 - 1/100.
IP Use at an assay dependent concentration.

Use at an assay dependent dilution.

ICC/IF Use at an assay dependent concentration. PubMed: 17971127

Use at an assay dependent dilution

ELISA 1/20000.

靶标

  • 功能Transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'. Promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation. Involved in activated KRAS-mediated transcriptional activation of USP28 in colorectal cancer (CRC) cells (PubMed:24623306). Binds to the USP28 promoter in colorectal cancer (CRC) cells (PubMed:24623306).
  • 序列相似性Belongs to the bZIP family. Jun subfamily.
    Contains 1 bZIP (basic-leucine zipper) domain.
  • 翻译后修饰Ubiquitinated by the SCF(FBXW7), leading to its degradation. Ubiquitination takes place following phosphorylation, that promotes interaction with FBXW7.
    Phosphorylated by CaMK4 and PRKDC; phosphorylation enhances the transcriptional activity. Phosphorylated by HIPK3. Phosphorylated by DYRK2 at Ser-243; this primes the protein for subsequent phosphorylation by GSK3B at Thr-239. Phosphorylated at Thr-239, Ser-243 and Ser-249 by GSK3B; phosphorylation reduces its ability to bind DNA. Phosphorylated by PAK2 at Thr-2, Thr-8, Thr-89, Thr-93 and Thr-286 thereby promoting JUN-mediated cell proliferation and transformation. Phosphorylated by PLK3 following hypoxia or UV irradiation, leading to increase DNA-binding activity.
    Acetylated at Lys-271 by EP300.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Activator protein 1 antibody
    • AP 1 antibody
    • AP1 antibody
    • cJun antibody
    • Enhancer Binding Protein AP1 antibody
    • Jun Activation Domain Binding Protein antibody
    • JUN antibody
    • Jun oncogene antibody
    • JUN protein antibody
    • Jun proto oncogene antibody
    • JUN_HUMAN antibody
    • JUNC antibody
    • Oncogene JUN antibody
    • p39 antibody
    • Proto oncogene c jun antibody
    • Proto oncogene cJun antibody
    • Proto-oncogene c-jun antibody
    • Transcription Factor AP 1 antibody
    • Transcription factor AP-1 antibody
    • Transcription Factor AP1 antibody
    • V jun avian sarcoma virus 17 oncogene homolog antibody
    • V jun sarcoma virus 17 oncogene homolog (avian) antibody
    • V jun sarcoma virus 17 oncogene homolog antibody
    • V-jun avian sarcoma virus 17 oncogene homolog antibody
    • vJun Avian Sarcoma Virus 17 Oncogene Homolog antibody
    see all

Anti-c-Jun (phospho S243) antibody 图像

  • All lanes : Anti-c-Jun (phospho S243) antibody (ab28846) at 1/500 dilution

    Lane 1 : HeLa cell lysate (5-30ug) - untreated
    Lane 2 : HeLa cell lysate (5-30ug) - treated with UV light


    Predicted band size : 36 kDa
    Observed band size : 39 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 48 kDa. We are unsure as to the identity of these extra bands.
  • Ab28846, at a dilution of 1/50, staining c-Jun in paraffin embedded human breast carcinoma by Immunohistochemistry.
    Left image: using c-Jun (phospho S243).
    Right image: the same antibody preincubated with the corresponding synthesized phosphopeptide.
    Ab28846, at a dilution of 1/50, staining c-Jun in paraffin embedded human breast carcinoma by Immunohistochemistry.
    Left image: using c-Jun (phospho S243).
    Right image: the same antibody preincubated with the corresponding synthesized phosphopeptide.

Anti-c-Jun (phospho S243) antibody (ab28846)参考文献

This product has been referenced in:
  • Bienemann AS  et al. Hsp70 suppresses apoptosis in sympathetic neurones by preventing the activation of c-Jun. J Neurochem 104:271-8 (2008). ICC/IF ; Rat . Read more (PubMed: 17971127) »

See 1 Publication for this product

Product Wall

There are currently no Abreviews or Questions for ab28846.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"