Anti-c-Jun抗体(ab5795)

Abreviews (1)Q&A (2)

概述

  • 产品名称
    Anti-c-Jun抗体
    参阅全部 c-Jun 一抗
  • 描述
    兔多克隆抗体to c-Jun
  • 宿主
    Rabbit
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Mouse
    预测可用于: Rat, Sheep, Chicken, Cow, Human, Pig, Xenopus laevis
  • 免疫原

    Synthetic peptide corresponding to Mouse c-Jun aa 91-105.
    Sequence:

    TPTPTQFLCPKNVTD

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • 阳性对照
    • NIH 3T3 cells.

性能

应用

Our Abpromise guarantee covers the use of ab5795 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 10 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 39.7 kDa).

靶标

  • 功能
    Transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'. Promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation. Involved in activated KRAS-mediated transcriptional activation of USP28 in colorectal cancer (CRC) cells (PubMed:24623306). Binds to the USP28 promoter in colorectal cancer (CRC) cells (PubMed:24623306).
  • 序列相似性
    Belongs to the bZIP family. Jun subfamily.
    Contains 1 bZIP (basic-leucine zipper) domain.
  • 翻译后修饰
    Ubiquitinated by the SCF(FBXW7), leading to its degradation. Ubiquitination takes place following phosphorylation, that promotes interaction with FBXW7.
    Phosphorylated by CaMK4 and PRKDC; phosphorylation enhances the transcriptional activity. Phosphorylated by HIPK3. Phosphorylated by DYRK2 at Ser-243; this primes the protein for subsequent phosphorylation by GSK3B at Thr-239. Phosphorylated at Thr-239, Ser-243 and Ser-249 by GSK3B; phosphorylation reduces its ability to bind DNA. Phosphorylated by PAK2 at Thr-2, Thr-8, Thr-89, Thr-93 and Thr-286 thereby promoting JUN-mediated cell proliferation and transformation. Phosphorylated by PLK3 following hypoxia or UV irradiation, leading to increase DNA-binding activity.
    Acetylated at Lys-271 by EP300.
  • 细胞定位
    Nucleus.
  • Information by UniProt
  • 数据库链接
    see all
  • 别名
    • Activator protein 1 antibody
    • AP 1 antibody
    • AP1 antibody
    • cJun antibody
    • Enhancer Binding Protein AP1 antibody
    • Jun Activation Domain Binding Protein antibody
    • JUN antibody
    • Jun oncogene antibody
    • JUN protein antibody
    • Jun proto oncogene antibody
    • JUN_HUMAN antibody
    • JUNC antibody
    • Oncogene JUN antibody
    • p39 antibody
    • Proto oncogene c jun antibody
    • Proto oncogene cJun antibody
    • Proto-oncogene c-jun antibody
    • Transcription Factor AP 1 antibody
    • Transcription factor AP-1 antibody
    • Transcription Factor AP1 antibody
    • V jun avian sarcoma virus 17 oncogene homolog antibody
    • V jun sarcoma virus 17 oncogene homolog (avian) antibody
    • V jun sarcoma virus 17 oncogene homolog antibody
    • V-jun avian sarcoma virus 17 oncogene homolog antibody
    • vJun Avian Sarcoma Virus 17 Oncogene Homolog antibody
    see all

实验方案

文献

ab5795 has not yet been referenced specifically in any publications.

发表研究结果有使用 ab5795？请让我们知道，以便我们可以引用本数据表中的参考文章。

客户评价及客户问答

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3 reviews or Q&A

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ChIP abreview for Anti-c-Jun antibody

 Average
Abreviews
Application
ChIP
Sample
Mouse Tissue lysate - whole (skin epidermis)
Specification
skin epidermis
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: 1% formaldehyde
Detection step
Semiquantitative PCR
Positive control
Untreated and TPA treated samples were used. Only in the case of TPA treated sample, a specific signal was obtained. Another c-jun antibody from a different company, which we had tested earlier and obtained positive result, was also included in parallel. In the case of both Abcam and the other companies antibodies, specific signal was obtained only when the skin was treated with TPA. However, the other companies antibody was more efficient in immunoprecipitation in comparison to Abcam antibody.
Negative control
A region corresponding to the coding sequence of the gene, which does not reveal any c-jun binding site by bioinformatics analysis, was used for PCR amplification. However, no amplification was observed.
Also there was no binding in the absence of TPA as mentioned above.
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Username

Dr. milan surjit

Verified customer

提交于 Sep 21 2007

We recently purchased the above antibody (ab5795; anti-cJun) and are using it in Western blots. We have two specific questions: (1) Does it cross react with other members of the Jun family (eg. JunD, JunB)? (2) Does it pick up both phosphorylated an...

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Below is the information that we have for ab5795: Ab5795 detects cJun from mouse cells. Using the sequence information (TPTPTQFLCPKNVTD) you can run a BLAST search on Genbank to determine if cross-reactivity would take place. The originator has not...

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The NIH 3T3 serum stimulated cells that were used - what is the protocol that was used to stimulate the cells?

Our material/methods says that it was 20% serum stimulated for 2hrs at physiological temperature. If you have any more questions, please do let us know.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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