概述

  • 产品名称Anti-c-Jun抗体
    参阅全部 c-Jun 一抗
  • 描述
    兔多克隆抗体to c-Jun
  • 特异性ab31367 detects endogenous levels of c-Jun protein around Serine 73.
  • 经测试应用适用于: IHC-Fr, WB, IHC-P, ELISAmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to Human c-Jun. Range of aa 40-89 Synthetic non-phosphopeptide derived from human c-Jun around the phosphorylation site of Serine 73.
    Database link: P05412

  • 阳性对照
    • IHC: breast carcinoma WB: HeLa cells extract

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab31367 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr Use at an assay dependent concentration. PubMed: 22615549
WB 1/500 - 1/1000. Detects a band of approximately 42 kDa (predicted molecular weight: 36 kDa).
IHC-P Use at an assay dependent concentration.
ELISA 1/10000.

靶标

  • 功能Transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'. Promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation. Involved in activated KRAS-mediated transcriptional activation of USP28 in colorectal cancer (CRC) cells (PubMed:24623306). Binds to the USP28 promoter in colorectal cancer (CRC) cells (PubMed:24623306).
  • 序列相似性Belongs to the bZIP family. Jun subfamily.
    Contains 1 bZIP (basic-leucine zipper) domain.
  • 翻译后修饰Ubiquitinated by the SCF(FBXW7), leading to its degradation. Ubiquitination takes place following phosphorylation, that promotes interaction with FBXW7.
    Phosphorylated by CaMK4 and PRKDC; phosphorylation enhances the transcriptional activity. Phosphorylated by HIPK3. Phosphorylated by DYRK2 at Ser-243; this primes the protein for subsequent phosphorylation by GSK3B at Thr-239. Phosphorylated at Thr-239, Ser-243 and Ser-249 by GSK3B; phosphorylation reduces its ability to bind DNA. Phosphorylated by PAK2 at Thr-2, Thr-8, Thr-89, Thr-93 and Thr-286 thereby promoting JUN-mediated cell proliferation and transformation. Phosphorylated by PLK3 following hypoxia or UV irradiation, leading to increase DNA-binding activity.
    Acetylated at Lys-271 by EP300.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Activator protein 1 antibody
    • AP 1 antibody
    • AP1 antibody
    • cJun antibody
    • Enhancer Binding Protein AP1 antibody
    • Jun Activation Domain Binding Protein antibody
    • JUN antibody
    • Jun oncogene antibody
    • JUN protein antibody
    • Jun proto oncogene antibody
    • JUN_HUMAN antibody
    • JUNC antibody
    • Oncogene JUN antibody
    • p39 antibody
    • Proto oncogene c jun antibody
    • Proto oncogene cJun antibody
    • Proto-oncogene c-jun antibody
    • Transcription Factor AP 1 antibody
    • Transcription factor AP-1 antibody
    • Transcription Factor AP1 antibody
    • V jun avian sarcoma virus 17 oncogene homolog antibody
    • V jun sarcoma virus 17 oncogene homolog (avian) antibody
    • V jun sarcoma virus 17 oncogene homolog antibody
    • V-jun avian sarcoma virus 17 oncogene homolog antibody
    • vJun Avian Sarcoma Virus 17 Oncogene Homolog antibody
    see all

Anti-c-Jun antibody 图像

  • ab31367 at 1:100, Immunohistochemical analysis of paraffin-embedded breast carcinoma. Left: c-Jun antibody, Right: Preincubated with synthesized peptide.
  • All lanes : Anti-c-Jun antibody (ab31367) at 1/1000 dilution

    Lane 1 : HeLa cells lysate, preincubated with immunogen (synthetic non-phosphopeptide)
    Lane 2 : HeLa cells lysate


    Predicted band size : 36 kDa

Anti-c-Jun antibody (ab31367)参考文献

This product has been referenced in:
  • Winter C  et al. Google goes cancer: improving outcome prediction for cancer patients by network-based ranking of marker genes. PLoS Comput Biol 8:e1002511 (2012). IHC-Fr ; Human . Read more (PubMed: 22615549) »
  • Samson WK  et al. Neuronostatin encoded by the somatostatin gene regulates neuronal, cardiovascular, and metabolic functions. J Biol Chem 283:31949-59 (2008). IHC-P ; Mouse . Read more (PubMed: 18753129) »

See all 2 Publications for this product

Product Wall

There are currently no Abreviews or Questions for ab31367.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"