概述

  • 产品名称Anti-c-IAP1 and c-IAP2抗体
  • 描述
    兔多克隆抗体to c-IAP1 and c-IAP2
  • 特异性This antibody detects both c-IAP1 and c-IAP2.
  • 经测试应用适用于: ICC/IF, WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide corresponding to 14 C terminal amino acids of Human cIAP1

  • 阳性对照
    • Human lung cell lysate can be used as positive control.

性能

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应用

Our Abpromise guarantee covers the use of ab25939 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 70 kDa.
IHC-P Use a concentration of 10 µg/ml.

Anti-c-IAP1 and c-IAP2 antibody 图像

  • Lane 1 : Anti-c-IAP1 and c-IAP2 antibody (ab25939) at 1 µg/ml
    Lane 2 : Anti-c-IAP1 and c-IAP2 antibody (ab25939) at 2 µg/ml
    Lane 3 : Anti-c-IAP1 and c-IAP2 antibody (ab25939) at 4 µg/ml

    Lane 1 : human lung lysate
    Lane 2 : human lung lysate
    Lane 3 : human lung lysate
  • Lane 1 : Anti-c-IAP1 and c-IAP2 antibody (ab25939) at 1 µg/ml
    Lane 2 : Anti-c-IAP1 and c-IAP2 antibody (ab25939) at 2 µg/ml
    Lane 3 : Anti-c-IAP1 and c-IAP2 antibody (ab25939) at 4 µg/ml

    Lane 1 : Human lung lysate
    Lane 2 : Human lung lysate
    Lane 3 : Human lung lysate
  • ab25939 at 10µg/ml staining cIAP1 in human lung cells by IHC 

  • ICC/IF image of ab25939 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab25939, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Immunofluorescence of cIAP in Human Lung cells using ab25939 at 20 ug/ml.

Anti-c-IAP1 and c-IAP2 antibody (ab25939)参考文献

This product has been referenced in:
  • Gualco E  et al. IGF-IR-dependent expression of Survivin is required for T-antigen-mediated protection from apoptosis and proliferation of neural progenitors. Cell Death Differ 17:439-51 (2010). IHC-P ; Mouse . Read more (PubMed: 19834489) »
  • Zhao TT  et al. hnRNP A1 regulates UV-induced NF-kappaB signalling through destabilization of cIAP1 mRNA. Cell Death Differ : (2008). WB ; Human . Read more (PubMed: 18846111) »

See all 2 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HEK293)
Loading amount 25 µg
Specification HEK293
Gel Running Conditions Non-reduced Denaturing (10% gel)
Blocking step Milk as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 21°C
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提交于 Jul 23 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"