Anti-c Abl (phospho Y412)抗体(ab55284)


  • 产品名称Anti-c Abl (phospho Y412)抗体
    参阅全部 c Abl 一抗
  • 描述
    兔多克隆抗体to c Abl (phospho Y412)
  • 特异性ab55284 detects endogenous levels of Abl1 only when phosphorylated at tyrosine 412.
  • 经测试应用适用于: ELISA, WBmore details
  • 种属反应性
    与反应: African Green Monkey
    预测可用于: Mouse, Rat, Human
  • 免疫原

    Synthetic phosphopeptide derived from human Abl1 around the phosphorylation site of tyrosine 412 (D-T-YP-T-A). Note: Two forms of c Abl are generated by alternative splicing. Tyr residue at position 412 is present in isoforms 1b of c Abl. The corresponding phosphorylation site in isoforms 1a is at position 393.

  • 阳性对照
    • extracts from COS7 cells, treated with Adriamycin




Our Abpromise guarantee covers the use of ab55284 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
  • 应用说明ELISA: 1/5000.
    WB: 1/500 - 1/1000. Detects a band of approximately 123 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Protein kinase that regulates key processes linked to cell growth and survival. Regulates cytoskeleton remodeling during cell differentiation, cell division and cell adhesion. Localizes to dynamic actin structures, and phosphorylates CRK and CRKL, DOK1, and other proteins controlling cytoskeleton dynamics. Regulates DNA repair potentially by activating the proapoptotic pathway when the DNA damage is too severe to be repaired. Phosphorylates PSMA7 that leads to an inhibition of proteasomal activity and cell cycle transition blocks.
    • 组织特异性Widely expressed.
    • 疾病相关Note=A chromosomal aberration involving ABL1 is a cause of chronic myeloid leukemia. Translocation t(9;22)(q34;q11) with BCR. The translocation produces a BCR-ABL found also in acute myeloid leukemia (AML) and acute lymphoblastic leukemia (ALL).
    • 序列相似性Belongs to the protein kinase superfamily. Tyr protein kinase family. ABL subfamily.
      Contains 1 protein kinase domain.
      Contains 1 SH2 domain.
      Contains 1 SH3 domain.
    • 翻译后修饰Phosphorylated by PRKDC (By similarity). DNA damage-induced activation of c-Abl requires the function of ATM and Ser-446 phosphorylation (By similarity). Phosphorylation on Thr-735 is required for binding 14-3-3 proteins for cytoplasmic translocation.
      Isoform IB is myristoylated on Gly-2.
    • 细胞定位Cytoplasm > cytoskeleton. Nucleus. Sequestered into the cytoplasm through interaction with 14-3-3 proteins and Nucleus membrane. The myristoylated c-ABL protein is reported to be nuclear.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Abelson murine leukemia viral oncogene homolog 1 antibody
      • Abelson tyrosine protein kinase 1 antibody
      • Abl 1 antibody
      • ABL antibody
      • ABL proto oncogene 1 non receptor tyrosine kinase antibody
      • ABL1 antibody
      • ABL1_HUMAN antibody
      • bcr/abl antibody
      • bcr/c abl oncogene protein antibody
      • c ABL antibody
      • c abl oncogene 1 non receptor tyrosine kinase antibody
      • c abl oncogene 1 receptor tyrosine kinase antibody
      • c ABL1 antibody
      • JTK7 antibody
      • p150 antibody
      • Proto oncogene tyrosine protein kinase ABL1 antibody
      • Proto-oncogene c-Abl antibody
      • Tyrosine-protein kinase ABL1 antibody
      • v abl Abelson murine leukemia viral oncogene homolog 1 antibody
      • v abl antibody
      see all

    Anti-c Abl (phospho Y412) antibody 图像

    • All lanes : Anti-c Abl (phospho Y412) antibody (ab55284) at 1/500 dilution

      Lane 1 : extracts from COS7 cells, treated with Adriamycin (0.5ng/ml, 24 hours).
      Lane 2 : extracts from COS7 cells, treated with Adriamycin (0.5ng/ml, 24 hours); ab55284 pre-incubated with the immunising phosphopeptide.

      Lysates/proteins at 5 µg per lane.

      Goat Anti-Rabbit IgG (H+L)

      Predicted band size : 123 kDa
      Observed band size : 123 kDa

    Anti-c Abl (phospho Y412) antibody (ab55284)参考文献

    ab55284 has not yet been referenced specifically in any publications.

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