Anti-c Abl (phospho Y412)抗体(ab47315)

概述

  • 产品名称
    Anti-c Abl (phospho Y412)抗体
    参阅全部 c Abl 一抗
  • 描述
    兔多克隆抗体to c Abl (phospho Y412)
  • 特异性
    ab47315 detects endogenous levels of c-Abl only when phosphorylated at tyrosine 412.
  • 经测试应用
    适用于: ICC/IF, WB, IHC-P, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    The antiserum was produced against synthesized phosphopeptide derived from human c-Abl around the phosphorylation site of tyrosine 412 (D-T-YP-T-A). Note: Two forms of c Abl are generated by alternative splicing. Tyr residue at position 412 is present in isoforms 1b of c Abl. The corresponding phosphorylation site in isoforms 1a is at position 393.

  • 阳性对照
    • Human breast carcinoma tissue

性能

应用

Our Abpromise guarantee covers the use of ab47315 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/100.
WB Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
ELISA 1/4000.

靶标

  • 功能
    Protein kinase that regulates key processes linked to cell growth and survival. Regulates cytoskeleton remodeling during cell differentiation, cell division and cell adhesion. Localizes to dynamic actin structures, and phosphorylates CRK and CRKL, DOK1, and other proteins controlling cytoskeleton dynamics. Regulates DNA repair potentially by activating the proapoptotic pathway when the DNA damage is too severe to be repaired. Phosphorylates PSMA7 that leads to an inhibition of proteasomal activity and cell cycle transition blocks.
  • 组织特异性
    Widely expressed.
  • 疾病相关
    Note=A chromosomal aberration involving ABL1 is a cause of chronic myeloid leukemia. Translocation t(9;22)(q34;q11) with BCR. The translocation produces a BCR-ABL found also in acute myeloid leukemia (AML) and acute lymphoblastic leukemia (ALL).
  • 序列相似性
    Belongs to the protein kinase superfamily. Tyr protein kinase family. ABL subfamily.
    Contains 1 protein kinase domain.
    Contains 1 SH2 domain.
    Contains 1 SH3 domain.
  • 翻译后修饰
    Phosphorylated by PRKDC (By similarity). DNA damage-induced activation of c-Abl requires the function of ATM and Ser-446 phosphorylation (By similarity). Phosphorylation on Thr-735 is required for binding 14-3-3 proteins for cytoplasmic translocation.
    Isoform IB is myristoylated on Gly-2.
  • 细胞定位
    Cytoplasm > cytoskeleton. Nucleus. Sequestered into the cytoplasm through interaction with 14-3-3 proteins and Nucleus membrane. The myristoylated c-ABL protein is reported to be nuclear.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Abelson murine leukemia viral oncogene homolog 1 antibody
    • Abelson tyrosine protein kinase 1 antibody
    • Abl 1 antibody
    • ABL antibody
    • ABL proto oncogene 1 non receptor tyrosine kinase antibody
    • ABL1 antibody
    • ABL1_HUMAN antibody
    • bcr/abl antibody
    • bcr/c abl oncogene protein antibody
    • c ABL antibody
    • c abl oncogene 1 non receptor tyrosine kinase antibody
    • c abl oncogene 1 receptor tyrosine kinase antibody
    • c ABL1 antibody
    • JTK7 antibody
    • p150 antibody
    • Proto oncogene tyrosine protein kinase ABL1 antibody
    • Proto-oncogene c-Abl antibody
    • Tyrosine-protein kinase ABL1 antibody
    • v abl Abelson murine leukemia viral oncogene homolog 1 antibody
    • v abl antibody
    see all

Anti-c Abl (phospho Y412) antibody 图像

  • ab47315 staining human breast carcinoma by IHC-P (left hand panel). The right hand panel shows staining in the presence of phospho-peptide.


  • Performed under reducing conditions.

    Exposure time : 30 seconds

    See Abreview

  • ab47315 staining c Abl (phospho Y412) in human platelets by Immunocytochemistry/ Immunofluorescence. Cells were fixed with formaldehyde and permeabilized with 0.2% Triton x100 before blocking with 10% serum at 200C for 1 hour. Samples were incubated with primary antibody (1/100: in 10% goat serum) for 1 hour 20 minutes at 20°C. An Alexa Fluor®488-conjugated goat polyclonal to rabbit IgG used at dilution at 1/400 as secondary antibody.

    See Abreview

Anti-c Abl (phospho Y412) antibody (ab47315)参考文献

This product has been referenced in:
  • Kundu P  et al. An EphB-Abl signaling pathway is associated with intestinal tumor initiation and growth. Sci Transl Med 7:281ra44 (2015). Read more (PubMed: 25834110) »
  • Xu J  et al. c-Abl mediates endothelial apoptosis induced by inhibition of integrins alphavbeta3 and alphavbeta5 and by disruption of actin. Blood 115:2709-18 (2010). WB ; Human . Read more (PubMed: 20124512) »

See all 3 Publications for this product

Product Wall

Thank you for contacting us with your enquiry. Sometimes we areable to provide antibodies in custom buffers, however we do require that a bulk volume (10 or more vials) are purchased in one order. If you are interested in purchasing a bulk volume, I ca...

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Application
Western blot
Sample
Mouse Cell lysate - whole cell (platelet)
Loading amount
20 µg
Specification
platelet
Treatment
with or without MnCl2 20 minutes
Gel Running Conditions
Reduced Denaturing (7.5 % polyacrylamide)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 22°C
Username

Dr. Ana Kasirer-Friede

Verified customer

提交于 Feb 16 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (platelet)
Specification
platelet
Fixative
Formaldehyde
Permeabilization
Yes - 0.2% Triton X-100
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Username

Abcam user community

Verified customer

提交于 Jun 22 2009

The peptide pair ab5774 (one peptide is phosphorylated, the other is not), is not identical to the peptide used to generate antibody ab47315; it is a little bigger, approximately 10 amino acids according to our source. But it does contain the sequence ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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