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The antiserum was produced against synthesized phosphopeptide derived from human c-Abl around the phosphorylation site of tyrosine 412 (D-T-YP-T-A). Note: Two forms of c Abl are generated by alternative splicing. Tyr residue at position 412 is present in isoforms 1b of c Abl. The corresponding phosphorylation site in isoforms 1a is at position 393.
Our Abpromise guarantee covers the use of ab47315 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration.|
|IHC-P||Use at an assay dependent concentration.|
ab47315 staining c Abl (phospho Y412) in human platelets by Immunocytochemistry/ Immunofluorescence. Cells were fixed with formaldehyde and permeabilized with 0.2% Triton x100 before blocking with 10% serum at 200C for 1 hour. Samples were incubated with primary antibody (1/100: in 10% goat serum) for 1 hour 20 minutes at 20°C. An Alexa Fluor®488-conjugated goat polyclonal to rabbit IgG used at dilution at 1/400 as secondary antibody.