Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (BJAB)
Total protein in input
10 µg
Immuno-precipitation step
Protein A/G
Specification
BJAB
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C
Concentration
5 µl/mg lysate
Additional data
Additional Notes
Volume of antibody provided by the company was less than the nominal volume: 90 uL instead of 100 uL. Vial was briefly centrifuged before opening so there was no volume left on the cap or walls of the tube. This happened with 3 vials of antibody purchased so I suspect this is routine practice at abcam. However, the quality of the antibody is excellent.
Abcam response
Thank you for your feedback. I am sorry to hear you received vials with shortages. We aim to err on the side of a little too much antibody than not enough. Our in house quality checks involve us checking thousands of vials each month to ensure we aliquot the correct volume. We do recommend a thorough spin down to make sure no antibody is stuck in the grooves of the cap. If you receive a vial with lesser volume, we are happy to replace it for you under our Abpromise guarantee-just give us a call or email.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Meztli Arguello
Verified customer
提交于 Jul 11 2016