SureLight® APC Anti-Myc tag抗体[9E10] (ab72580)
Key features and details
- SureLight® APC Mouse monoclonal [9E10] to Myc tag
- Suitable for: Flow Cyt, FRET
- Conjugation: SureLight® APC. Ex: 652nm, Em: 657nm
- Isotype: IgG1
Related conjugates and formulations
概述
-
产品名称
SureLight® APC Anti-Myc tag抗体[9E10]
参阅全部 Myc tag 一抗 -
描述
SureLight® APC小鼠单克隆抗体[9E10] to Myc tag -
宿主
Mouse -
偶联物
SureLight® APC. Ex: 652nm, Em: 657nm -
特异性
This antibody is specific for Myc tagged proteins. The Myc tag epitope (EQKLISEEDL) is located at the dimerization site of c-myc and therefore this antibody does not perform well at recognizing endogenous c-myc. -
经测试应用
适用于: Flow Cyt, FRETmore details -
免疫原
Synthetic peptide corresponding to Human Myc tag aa 400 to the C-terminus conjugated to keyhole limpet haemocyanin.
-
表位
Epitope located at aa 410-419; EQKLISEEDL -
常规说明
Excitation max. 652 nm. Emission max. 657.5 nm. F/P 1:1.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. Store In the Dark. -
存储溶液
pH: 8.20
Preservative: 0.013% Sodium azide
Constituents: 1.64% Sodium phosphate, 0.1% BSA, 0.87% Sodium chloride, 97.37% Tris buffered saline -
Concentration information loading...
-
纯化说明
Size exclusion chromatography as well as some proprietary methods for purifying the final materials were used. -
克隆
单克隆 -
克隆编号
9E10 -
同种型
IgG1 -
研究领域
相关产品
-
Alternative Versions
- DyLight® 650 Anti-Myc tag antibody [9E10] (ab117487)
- DyLight® 488 Anti-Myc tag antibody [9E10] (ab117499)
- DyLight® 550 Anti-Myc tag antibody [9E10] (ab117511)
- FITC Anti-Myc tag antibody [9E10] (ab117599)
- Biotin Anti-Myc tag antibody [9E10] - C-terminal (ab197139)
- Alexa Fluor® 488 Anti-Myc tag antibody [9E10] (ab202008)
- Anti-Myc tag antibody [9E10] (ab206486)
- Alexa Fluor® 594 Anti-Myc tag antibody [9E10] (ab223894)
- Alexa Fluor® 647 Anti-Myc tag antibody [9E10] (ab223895)
- APC Anti-Myc tag antibody [9E10] (ab223896)
- Anti-Myc tag antibody [9E10] - BSA and Azide free (ab264603)
- Anti-Myc tag antibody [9E10] (ab32)
- HRP Anti-Myc tag antibody [9E10] (ab62928)
- PE Anti-Myc tag antibody [9E10] (ab72468)
- Biotin Anti-Myc tag antibody [9E10] (ab81658)
-
Assay kits
-
Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab72580于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
Flow Cyt |
Use at an assay dependent concentration.
ab73711 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
|
FRET |
Use at an assay dependent concentration.
|
说明 |
---|
Flow Cyt
Use at an assay dependent concentration. ab73711 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
FRET
Use at an assay dependent concentration. |
靶标
-
相关性
Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells. -
细胞定位
Nuclear -
别名
- c-myc tag antibody
- Myc Epitope Tag antibody
数据表及文件
-
SDS download
-
Datasheet download
文献 (2)
ab72580 被引用在 2 文献中.
- Hörnström D et al. Molecular optimization of autotransporter-based tyrosinase surface display. Biochim Biophys Acta Biomembr 1861:486-494 (2019). PubMed: 30521785
- Gustavsson M et al. Surface expression of ?-transaminase in Escherichia coli. Appl Environ Microbiol 80:2293-8 (2014). PubMed: 24487538