Application
Western blot
Sample
Human Cell lysate - whole cell (293T cells)
Loading amount
20 µg
Specification
293T cells
Gel Running Conditions
Reduced Denaturing (Invitrogen NUPAGE Bis-Tris Gel 4-12% Gradient Gel MES Buffer)
Blocking step
Milk as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Dilution
1/50000
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBST (0.2% Tween20) containing 5% milk
Secondary antibody
Secondary antibody
None used
Detection
Detection method
Pico and Femto ECL
Exposure
10 second(s)
Bands
Specific: 104 kDa
Positive control
Mouse RFX4 tagged with C-terminal myc tag in pCAG plasmid
Negative control
293T cells transfected with EGFP
Additional data
Additional Notes
I detected Mouse RFX1 tagged with C-terminal myc tag in pCAG plasmid using this antibody.
Extremely clean band. No non-specific bands. I love it.
Extremely clean band. No non-specific bands. I love it.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Mar 25 2010