Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (HeLa)
Total protein in input
20 µg
Immuno-precipitation step
Other - Magnetic Protein G
Specification
HeLa
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: NTEN Buffer
Concentration
1 µg/mg lysate
Additional data
Additional Notes
IP perfomed with cells expressin the protein TOPBP1 tagged with myc.
Lane 1: Input:
Lane 2: IgG
Lane 3: IP using ab32
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Jul 15 2013