Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Testis, postnatal day 6)
Permeabilization
Yes - 0.1% Triton X-100 in PBS
Specification
Testis, postnatal day 6
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
Fixative
Paraformaldehyde
Other product details
Incubation time
30 minute(s) · Temperature: 20°C
Dilution
1/250
Secondary antibody
Dilution
1/500
Name
Abcam antibody:Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed
Additional data
Additional Notes
Sections were counterstained with phalloidin (red), which labels F-actin.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MR. Bryan Niedenberger
Verified customer
提交于 Mar 08 2016