Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (testis)
Specification
testis
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 2% · Temperature: 20°C
Other product details
Dilution
1/100
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 2% BSA, 0.005 % Triton in PBS
Secondary antibody
Secondary antibody
None used
Additional data
Additional Notes
Very good ab in a rare species (rat) very useful for multicolor IHC. Image description: mouse testis, acquired with a Zeiss 510 LSM confocal laser scanning microscope, a 3 D image projection from a Z-stack of 25 slides is shown. From left to right: anti Oct4 (green colour) DAPI (blue) anti-c-Kit (red) DIC (grey) Merge
Abcam response
avidin coupled to a fluorophore was used for detection
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Feb 24 2011