Anti-c-Jun (phospho T91 + T93)抗体[C-J 4C4] (ab13671)
Key features and details
- Mouse monoclonal [C-J 4C4] to c-Jun (phospho T91 + T93)
- Suitable for: IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG1
概述
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产品名称
Anti-c-Jun (phospho T91 + T93)抗体[C-J 4C4]
参阅全部 c-Jun 一抗 -
描述
小鼠单克隆抗体[C-J 4C4] to c-Jun (phospho T91 + T93) -
宿主
Mouse -
经测试应用
适用于: IHC-P, ICC/IFmore details -
种属反应性
与反应: Human -
免疫原
Recombinant full length protein corresponding to Human c-Jun (phospho T91 + T93).
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阳性对照
- This antibody gave a positive result in IHC in the following FFPE tissue: Human normal lung.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.02% Sodium azide
Constituent: 99.98% PBS -
Concentration information loading...
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纯度
Protein A/G purified -
克隆
单克隆 -
克隆编号
C-J 4C4 -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab13671于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P |
Use a concentration of 5 µg/ml.
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ICC/IF |
Use a concentration of 1 µg/ml.
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说明 |
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IHC-P
Use a concentration of 5 µg/ml. |
ICC/IF
Use a concentration of 1 µg/ml. |
靶标
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功能
Transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'. Promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation. Involved in activated KRAS-mediated transcriptional activation of USP28 in colorectal cancer (CRC) cells (PubMed:24623306). Binds to the USP28 promoter in colorectal cancer (CRC) cells (PubMed:24623306). -
序列相似性
Belongs to the bZIP family. Jun subfamily.
Contains 1 bZIP (basic-leucine zipper) domain. -
翻译后修饰
Ubiquitinated by the SCF(FBXW7), leading to its degradation. Ubiquitination takes place following phosphorylation, that promotes interaction with FBXW7.
Phosphorylated by CaMK4 and PRKDC; phosphorylation enhances the transcriptional activity. Phosphorylated by HIPK3. Phosphorylated by DYRK2 at Ser-243; this primes the protein for subsequent phosphorylation by GSK3B at Thr-239. Phosphorylated at Thr-239, Ser-243 and Ser-249 by GSK3B; phosphorylation reduces its ability to bind DNA. Phosphorylated by PAK2 at Thr-2, Thr-8, Thr-89, Thr-93 and Thr-286 thereby promoting JUN-mediated cell proliferation and transformation. Phosphorylated by PLK3 following hypoxia or UV irradiation, leading to increase DNA-binding activity.
Acetylated at Lys-271 by EP300. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 3725 Human
- Omim: 165160 Human
- SwissProt: P05412 Human
- Unigene: 696684 Human
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别名
- Activator protein 1 antibody
- AP 1 antibody
- AP-1 antibody
see all
图片
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ICC/IF image of ab13617 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab13617, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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IHC image of c-Jun (phospho T91 + T93) staining in Human normal lung formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab13671, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (6)
ab13671 被引用在 6 文献中.
- Hu G et al. MicroRNA-145 attenuates TNF-a-driven cartilage matrix degradation in osteoarthritis via direct suppression of MKK4. Cell Death Dis 8:e3140 (2017). IHC-P ; Human . PubMed: 29072705
- Kudo K et al. Inhibition of Gli1 results in altered c-Jun activation, inhibition of cisplatin-induced upregulation of ERCC1, XPD and XRCC1, and inhibition of platinum-DNA adduct repair. Oncogene 31:4718-24 (2012). Human . PubMed: 22266871
- Madeo A et al. c-Jun activation is required for 4-hydroxytamoxifen-induced cell death in breast cancer cells. Oncogene 29:978-91 (2010). WB ; Human . PubMed: 19935718
- Vinciguerra M et al. Negative charged threonine 95 of c-Jun is essential for c-Jun N-terminal kinase-dependent phosphorylation of threonine 91/93 and stress-induced c-Jun biological activity. Int J Biochem Cell Biol 40:307-16 (2008). PubMed: 17920329
- Hartz AM et al. Diesel exhaust particles induce oxidative stress, proinflammatory signaling, and P-glycoprotein up-regulation at the blood-brain barrier. FASEB J : (2008). PubMed: 18474546
- Nateri AS et al. The ubiquitin ligase SCFFbw7 antagonizes apoptotic JNK signaling. Science 303:1374-8 (2004). PubMed: 14739463