Application
IHC - Wholemount
Sample
Zebrafish Embryo (all embryo)
Specification
all embryo
Other product details
Dilution
1/100
Incubation time
22 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 10% goat serum in PBT
Secondary antibody
Name
Non-Abcam antibody was used: Alexa Fluor 568 goat anti—rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 568
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 568
Dilution
1/1000
Additional data
Additional Notes
Fixation: 24 hpf embryos were fixed with 4% PFA 48h 4 degrees (washed PBS x 2, washed methanol x 2) OR Dent's fixative 48h 4 degrees (washed methanol x2).
Permeabilization : 5 minutes MeOH
Blocked: 10% goat serum in PBT.
Permeabilization : 5 minutes MeOH
Blocked: 10% goat serum in PBT.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Mar 14 2012